CADM3 active protein

CADM3 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120736

• Gene Names: CADM3; BIgR; NECL1; TSLL1; IGSF4B; Necl-1; synCAM3
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CADM3; CADM3 Protein; Human; Recombinant (His Tag); Human CADM3/NECL1/IGSF4B Protein (His Tag); BIgR Protein; IGSF4B Protein; Necl-1 Protein; NECL1 Protein; synCAM3 Protein; TSLL1 Protein; cell adhesion molecule 3; CADM3 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-His330

• Species: Human
• Activity: Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. Human CADM3 immobilized (0.8ug/ml, 100uL/well) will mediate >30% C6 cell adhesion.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Asn 25
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CADM3 isoform 2 (short isoform)(NP_001120645.1) extracellular domain (Met 1-His 330) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for CADM3 active protein

Background: Cell Adhesion Molecules (CAMs) are proteins located on the cell surface involved with the binding with other cells or with the extracellular matrix (ECM) in the process called cell adhesion. These proteins are typically transmembrane receptors and are composed of three domains: an intracellular domain that interacts with the cytoskeleton, a transmembrane domain, and an extracellular domain that interacts either with other CAMs of the same kind (homophilic binding) or with other CAMs or the extracellular matrix (heterophilic binding). Cell adhesion molecule 3, also known as Immunoglobulin superfamily member 4B, CADM3, and NECL1, is a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule which has Ca(2+)-independent homo- or heterophilic cell-cell adhesion activity and plays an important role in the formation of synapses, axon bundles and myelinated axons. Isoform 1 of CADM3 is expressed mainly in adult and fetal brain. Isoform 2 of CADM3 is highly expressed in adult brain and weakly expressed in placenta. In brain, Isoform 2 is highly expressed in cerebellum. CADM3 is involved in the cell-cell adhesion. It has both calcium-independent homophilic cell-cell adhesion activity and calcium-independent heterophilic cell-cell adhesion activity with IGSF4, PVRL1 and PVRL3. The interaction with EPB41L1 may regulate structure or function of cell-cell junctions. CADM3 may act as a tumor suppressor in glioma and loss of it in glioma may be caused by histone deacetylation.

References

Dong X, et al. (2006) Crystal structure of the V domain of human Nectin-like molecule-1/Syncam3/Tsll1/Igsf4b, a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule. J Biol Chem. 281(15): 10610-7.Gao J, et al. (2008) Nectin-like molecule 1 is a glycoprotein with a single N-glycosylation site at N290KS which influences its adhesion activity. Biochim Biophys Acta. 1778(6): 1429-35.Gao J, et al. (2009) Loss of NECL1, a novel tumor suppressor, can be restored in glioma by HDAC inhibitor-Trichostatin A through Sp1 binding site. Glia. 57(9): 989-99.

NCBI and Uniprot Product Information

• NCBI GI #: 187608363
• NCBI GeneID: 57863
• NCBI Accession #: NP_001120645.1
• NCBI GenBank Nucleotide #: NM_001127173.1
• UniProt Accession #: Q8N126; Q8IZQ9; Q9NVJ5; Q9UJP1
• Molecular Weight: 43,300 Da
• NCBI Official Full Name: cell adhesion molecule 3 isoform 2
• NCBI Official Synonym Full Names: cell adhesion molecule 3
• NCBI Official Symbol: CADM3
• NCBI Official Synonym Symbols: BIgR; NECL1; TSLL1; IGSF4B; Necl-1; synCAM3
• NCBI Protein Information: cell adhesion molecule 3; TSLC1-like 1; nectin-like 1; TSLC1-like protein 1; nectin-like protein 1; brain immunoglobulin receptor; synaptic cell adhesion molecule 3; immunoglobulin superfamily member 4B; immunoglobulin superfamily, member 4B; dendritic ce
• UniProt Protein Name: Cell adhesion molecule 3
• Protein Family: Cell adhesion molecule
• UniProt Gene Name: CADM3
• UniProt Synonym Gene Names: IGSF4B; NECL1; SYNCAM3; TSLL1; IgSF4B; NECL-1; SynCAM3; TSLL1
• UniProt Entry Name: CADM3_HUMAN

NCBI Description

IGSF4B is a brain-specific protein related to the calcium-independent cell-cell adhesion molecules known as nectins (see PVRL3; MIM 607147) (Kakunaga et al., 2005 [PubMed 15741237]).[supplied by OMIM, Mar 2008]

Uniprot Description

CADM3: Involved in the cell-cell adhesion. Has both calcium- independent homophilic cell-cell adhesion activity and calcium- independent heterophilic cell-cell adhesion activity with IGSF4, PVRL1 and PVRL3. Interaction with EPB41L1 may regulate structure or function of cell-cell junctions. Belongs to the nectin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 1q21.2-q22

Cellular Component: integral to membrane; plasma membrane; intercellular junction

Molecular Function: protein homodimerization activity

Biological Process: heterophilic cell adhesion; intercellular junction assembly and maintenance; protein localization; homophilic cell adhesion