Rabbit anti-Human CADM2 Polyclonal Antibody | anti-CADM2 antibody

CADM2, NT (CADM2, IGSF4D, NECL3, Cell adhesion molecule 2, Immunoglobulin superfamily member 4D, Nectin-like protein 3, Synaptic cell adhesion molecule 2) (Azide free) (HRP)

Cat. Num. AAA6280456

• Gene Names: CADM2; NECL3; IGSF4D; Necl-3; synCAM2; SynCAM 2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CADM2; Polyclonal Antibody; NT (CADM2; IGSF4D; NECL3; Cell adhesion molecule 2; Immunoglobulin superfamily member 4D; Nectin-like protein 3; Synaptic cell adhesion molecule 2) (Azide free) (HRP); Synaptic cell adhesion molecule 2; anti-CADM2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CADM2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CADM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-76 amino acids from the N-terminal region of human CADM2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CADM2 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CADM2 antibody detected the CADM2 protein (arrow).)

Related Product Information for anti-CADM2 antibody

Members of the large immunoglobulin (Ig) superfamily, such as IGSF4D, have diverse roles in extracellular recognition and intercellular adhesion (Biederer, 2006 [PubMed 16311015]).[supplied by OMIM].

Product Categories/Family for anti-CADM2 antibody

Antibodies; Cell Markers

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Festen, E.A., et al. Am. J. Gastroenterol. 105(2):395-402(2010). Biederer, T. Genomics 87(1):139-150(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 264681533
• NCBI GeneID: 253559
• NCBI Accession #: NP_001161146.1; NP_001161147.1
• NCBI GenBank Nucleotide #: NM_001167674.1
• UniProt Accession #: Q8N3J6; Q3KQY9; Q658Q7; Q8IZP8
• Molecular Weight: 47,554 Da
• NCBI Official Full Name: cell adhesion molecule 2 isoform 1
• NCBI Official Synonym Full Names: cell adhesion molecule 2
• NCBI Official Symbol: CADM2
• NCBI Official Synonym Symbols: NECL3; IGSF4D; Necl-3; synCAM2; SynCAM 2
• NCBI Protein Information: cell adhesion molecule 2; nectin-like 3; nectin-like protein 3; synaptic cell adhesion molecule 2; immunoglobulin superfamily member 4D; immunoglobulin superfamily, member 4D
• UniProt Protein Name: Cell adhesion molecule 2
• Protein Family: Cell adhesion molecule
• UniProt Gene Name: CADM2
• UniProt Synonym Gene Names: IGSF4D; NECL3; IgSF4D; NECL-3; SynCAM 2
• UniProt Entry Name: CADM2_HUMAN

NCBI Description

This gene encodes a member of the synaptic cell adhesion molecule 1 (SynCAM) family which belongs to the immunoglobulin (Ig) superfamily. The encoded protein has three Ig-like domains and a cytosolic protein 4.1 binding site near the C-terminus. Proteins belonging to the protein 4.1 family crosslink spectrin and interact with other cytoskeletal proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

CADM2: Adhesion molecule that engages in homo- and heterophilic interactions with the other nectin-like family members, leading to cell aggregation. Important for synapse organization, providing regulated trans-synaptic adhesion. Preferentially binds to oligodendrocytes. Belongs to the nectin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p12.1

Cellular Component: axon; cytoplasm; plasma membrane; integral to membrane; synapse; cell junction

Biological Process: intercellular junction assembly and maintenance; brain development; cell adhesion