Rabbit anti-Mouse CADHERIN 13 Polyclonal Antibody | anti-CDH13 antibody

RABBIT ANTI HUMAN CADHERIN 13

Cat. Num. AAA221326

• Gene Names: CDH13; CDHH; P105
• Reactivity: Mouse
• Applications: Immunofluorescence, Western Blot
• Synonyms: CADHERIN 13; Polyclonal Antibody; RABBIT ANTI HUMAN CADHERIN 13; anti-CDH13 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises human Cadherin 13, a 78 kDa, calcium-dependent, glycoprotein. While Cadherin 13 is a member of the cadherin family, it lacks their conserved cytoplasmic region, being instead anchored to the plasma membrane via glycosylphosphatidylinositol linkage. It is expressed in the heart and CNS with the exception of the cerebellum and spinal cord. ; ; Cadherin 13 is classified as a cell-cell adhesion molecule, although it is likely that it's role is in signalling and not physical adhesion. It is involved in regulating low-density lipoprotein-initiated cell proliferation, keratinocyte proliferation, neural cell growth and may play a role in metabolic regulation. Cadherin 13 protects endothelial cells from oxidative stress-induced apoptosis and may contribute to progression of vascular disorders such as atherosclerosis, restenosis and tumour angiogenesis.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 674

• Applicable Applications for anti-CDH13 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band/s of approximately 78kDa in 3T3 cell lysates.; Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3)
• Immunogen: Synthetic peptide corresponding to N-terminus of human Cadherin 13.; Buffer Solution
• Antiserum Preparation: Antisera to Cadherin 13 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of whole cell lysates from NIH-3T3 mouse embryonic fibroblasts probed with Rabbit anti Human cadherin 13 (MBS221326) at 0.5(A) and 1(B) ug/ml)

Testing Data #2

(Immunofluorescence staining of K562 human erythromyeloblastoid cells with Rabbit anti Human cadherin 13 (MBS221326))

Related Product Information for anti-CDH13 antibody

MBS221326 recognises human Cadherin 13, a 78 kDa, calcium-dependent, glycoprotein. While Cadherin 13 is a member of the cadherin family, it lacks their conserved cytoplasmic region, being instead anchored to the plasma membrane via glycosylphosphatidylinositol linkage. It is expressed in the heart and CNS with the exception of the cerebellum and spinal cord. Cadherin 13 is classified as a cell-cell adhesion molecule, although it is likely that it's role is in signalling and not physical adhesion. It is involved in regulating low-density lipoprotein-initiated cell proliferation, keratinocyte proliferation, neural cell growth and may play a role in metabolic regulation. Cadherin 13 protects endothelial cells from oxidative stress-induced apoptosis and may contribute to progression of vascular disorders such as atherosclerosis, restenosis and tumour angiogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 333944018
• NCBI GeneID: 1012
• NCBI Accession #: NP_001207418.1
• NCBI GenBank Nucleotide #: NM_001220489.1
• UniProt Accession #: P55290; Q6GTW4; Q8TBX3; A8W476; A8W477; B7Z590; C9JRI6; J3KN62
• Molecular Weight: 73,787 Da
• NCBI Official Full Name: cadherin-13 isoform 3
• NCBI Official Synonym Full Names: cadherin 13
• NCBI Official Symbol: CDH13
• NCBI Official Synonym Symbols: CDHH; P105
• NCBI Protein Information: cadherin-13; H-cadherin (heart); T-cad; T-cadherin; cadherin 13, H-cadherin (heart); heart cadherin
• UniProt Protein Name: Cadherin-13
• Protein Family: Cadherin
• UniProt Gene Name: CDH13
• UniProt Synonym Gene Names: CDHH; H-cadherin; T-cad; T-cadherin
• UniProt Entry Name: CAD13_HUMAN

NCBI Description

This gene encodes a member of the cadherin superfamily. The encoded protein is localized to the surface of the cell membrane and is anchored by a GPI moiety, rather than by a transmembrane domain. The protein lacks the cytoplasmic domain characteristic of other cadherins, and so is not thought to be a cell-cell adhesion glycoprotein. This protein acts as a negative regulator of axon growth during neural differentiation. It also protects vascular endothelial cells from apoptosis due to oxidative stress, and is associated with resistance to atherosclerosis. The gene is hypermethylated in many types of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2011]

Uniprot Description

CDH13: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May act as a negative regulator of neural cell growth. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 16q23.3

Cellular Component: extracellular space; neuron projection; focal adhesion; cytoplasm; plasma membrane; caveola; external side of plasma membrane

Molecular Function: adiponectin binding; protein homodimerization activity; cadherin binding; low-density lipoprotein binding; calcium ion binding

Biological Process: lamellipodium biogenesis; intercellular junction assembly and maintenance; positive regulation of positive chemotaxis; positive regulation of smooth muscle cell proliferation; Rac protein signal transduction; positive regulation of calcium-mediated signaling; negative regulation of cell adhesion; keratinocyte proliferation; regulation of epidermal growth factor receptor signaling pathway; Rho protein signal transduction; low density lipoprotein mediated signaling; localization within membrane; negative regulation of cell proliferation; regulation of endocytosis; calcium-dependent cell-cell adhesion; endothelial cell migration; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; sprouting angiogenesis; homophilic cell adhesion; positive regulation of cell-matrix adhesion; positive regulation of cell migration