Rabbit CAD Polyclonal Antibody | anti-CAD antibody

CAD Antibody

Cat. Num. AAA9613423

• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (91%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (91%)
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: CAD; Polyclonal Antibody; CAD Antibody; Aspartate carbamoyltransferase; Aspartate transcarbamylase; CAD protein; CAD trifunctional protein; Carbamoyl phosphate synthetase 2; CPSase ATCase DHOase; Dihydroorotase; Glutamine dependent carbamoyl phosphate synthase; Multifunctional protein CAD; anti-CAD antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (91%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (91%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: CAD Antibody detects endogenous levels of total CAD.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CAD antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human CAD, corresponding to a region within C-terminal amino acids.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Activated by MAP kinase (Erk1/2) phosphorylation just prior to the S phase of the cell cycle, when the demand for pyrimidine nucleotides is greatest, and down-regulated as the cells emerge from S phase by protein kinase A (PKA) phosphorylation. Phosphorylation at Ser-1859 by RPS6KB1 downstream of MTOR promotes oligomerization and stimulates dihydroorotase activity. Phosphorylation at Ser-1406 reduces sensitivity to feedback inhibition by UTP.
• Subunit Structure: Homohexamer. Interacts with CIPC.
• Similarity: In the central section; belongs to the metallo-dependent hydrolases superfamily. DHOase family. CAD subfamily.
• Subcellular Location: Cytoplasm. Nucleus.; Note: Cytosolic and unphosphorylated in resting cells, translocates to the nucleus in response to EGF stimulation, nuclear import promotes optimal cell growth.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from various samples, using CAD Antibody.Lane 1: Colo205, blocked with antigen-specific peptides,Lane 2: Colo205,Lane 3: 293.)

Western Blot (WB)

(Western blot analysis of CAD in lysates of 293T  , using CAD Antibody(AF4715).)

Related Product Information for anti-CAD antibody

This protein is a 'fusion' protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).

NCBI and Uniprot Product Information

• NCBI GI #: 18105007
• NCBI GeneID: 790
• NCBI Accession #: NP_004332.2
• NCBI GenBank Nucleotide #: NM_004341.3
• UniProt Accession #: P27708; Q6P0Q0; D6W552
• Molecular Weight: 242,984 Da
• NCBI Official Full Name: CAD protein
• NCBI Official Synonym Full Names: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
• NCBI Official Symbol: CAD
• NCBI Protein Information: CAD protein; CAD trifunctional protein; multifunctional protein CAD
• UniProt Protein Name: CAD protein
• Protein Family: Cadherin
• UniProt Gene Name: CAD
• UniProt Entry Name: PYR1_HUMAN

NCBI Description

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. [provided by RefSeq, Jul 2008]

Uniprot Description

CAD: a multifunctional enzyme that catalyzes four enzymatic activities of the pyrimidine pathway, including the rate-limiting first step. The four enzymatic activities involved in de novo pyrimidine synthesis are glutamine amidotransferase, carbamoyl-phosphate synthetase, aspartate transcarbamoylase, and dihydroorotase activities. Provides essential precursors for DNA synthesis. Required for the de novo synthesis of pyrimidine nucleotides and cell growth. Phosphorylation by p70S6K promotes CAD oligomerization, stimulating de novo synthesis of pyrimidines and progression through S phase in mammalian cells. Utilizes glutamine, bicarbonate, and aspartic acid to form a pyrimidine ring that is subsequently attached to ribose to generate a pyrimidine.

Protein type: EC 6.3.5.5; EC 2.1.3.2; Transferase; Ligase; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 3.5.2.3; Hydrolase; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 2p22-p21

Cellular Component: nucleoplasm; cell projection; protein complex; nuclear matrix; membrane; cell soma; plasma membrane; terminal button; nucleus; cytosol

Molecular Function: carbamoyl-phosphate synthase (ammonia) activity; identical protein binding; enzyme binding; zinc ion binding; carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity; aspartate carbamoyltransferase activity; ATP binding; protein kinase activity; dihydroorotase activity

Biological Process: lactation; organ regeneration; arginine biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; protein amino acid autophosphorylation; heart development; UTP biosynthetic process; peptidyl-threonine phosphorylation; glutamine metabolic process; response to testosterone stimulus; female pregnancy; liver development; response to caffeine; 'de novo' pyrimidine base biosynthetic process; response to cortisol stimulus; pyrimidine base metabolic process; pyrimidine nucleoside biosynthetic process; glutamine catabolic process; drug metabolic process; urea cycle; response to amine stimulus

Disease: Congenital Disorder Of Glycosylation, Type Iz