Western Blot (WB)
(Western blot analysis of CAD expression in HeLa cell lysate.)
Rabbit anti-Human CAD Polyclonal Antibody | anti-CAD antibody
CAD Rabbit pAb
ICC/IF: 1:50-1:200
IP: 1:30
FC/FACS: 1:80
Western Blot (WB)
(Western blot analysis of CAD expression in HeLa cell lysate.)
Western Blot (WB)
(Western blot analysis of CAD expression in HeLa cell lysate.)
NCBI and Uniprot Product Information
NCBI Description
The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. [provided by RefSeq, Jul 2008]
Uniprot Description
CAD: a multifunctional enzyme that catalyzes four enzymatic activities of the pyrimidine pathway, including the rate-limiting first step. The four enzymatic activities involved in de novo pyrimidine synthesis are glutamine amidotransferase, carbamoyl-phosphate synthetase, aspartate transcarbamoylase, and dihydroorotase activities. Provides essential precursors for DNA synthesis. Required for the de novo synthesis of pyrimidine nucleotides and cell growth. Phosphorylation by p70S6K promotes CAD oligomerization, stimulating de novo synthesis of pyrimidines and progression through S phase in mammalian cells. Utilizes glutamine, bicarbonate, and aspartic acid to form a pyrimidine ring that is subsequently attached to ribose to generate a pyrimidine.
Protein type: EC 6.3.5.5; EC 2.1.3.2; Transferase; Ligase; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 3.5.2.3; Hydrolase; Nucleotide Metabolism - pyrimidine
Chromosomal Location of Human Ortholog: 2p22-p21
Cellular Component: nucleoplasm; cell projection; protein complex; nuclear matrix; membrane; cell soma; plasma membrane; terminal button; nucleus; cytosol
Molecular Function: carbamoyl-phosphate synthase (ammonia) activity; identical protein binding; enzyme binding; zinc ion binding; carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity; aspartate carbamoyltransferase activity; ATP binding; protein kinase activity; dihydroorotase activity
Biological Process: lactation; organ regeneration; arginine biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; protein amino acid autophosphorylation; heart development; UTP biosynthetic process; peptidyl-threonine phosphorylation; glutamine metabolic process; response to testosterone stimulus; female pregnancy; liver development; response to caffeine; 'de novo' pyrimidine base biosynthetic process; response to cortisol stimulus; pyrimidine base metabolic process; pyrimidine nucleoside biosynthetic process; glutamine catabolic process; drug metabolic process; urea cycle; response to amine stimulus
Disease: Congenital Disorder Of Glycosylation, Type Iz
Research Articles on CAD
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Product Notes
The CAD cad (Catalog #AAA4757334) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CAD Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CAD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:500-1:1000 ICC/IF: 1:50-1:200 IP: 1:30 FC/FACS: 1:80. Researchers should empirically determine the suitability of the CAD cad for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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