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Western Blot (WB) (Western Blot analysis of Carbonic anhydrase 9 on human lung lysates using anti- Carbonic anhydrase 9 antibody at 1/1,000 dilution.)

Rabbit anti-Human CA IX Polyclonal Antibody | anti-CAIX antibody

CA IX Polyclonal Antibody

Gene Names
CA9; MN; CAIX
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
ProA affinity purified
Synonyms
CA IX; Polyclonal Antibody; CA IX Polyclonal Antibody; CA-IX; CAIX; RCC-associated antigen G250; CA9; CAH9; Carbonate dehydratase IX; Carbonic anhydrase 9; Carbonic anhydrase IX; Carbonic dehydratase; G250; Membrane antigen MN; MN; P54/58N; pMW1; RCC associated protein G250; Renal cell carcinoma-associated antigen G250; anti-CAIX antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
CA IX polyclonal antibody detects endogenous levels of CA IX protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
459
Applicable Applications for anti-CAIX antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
Immunogen
recombinant protein
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Carbonic anhydrase 9 on human lung lysates using anti- Carbonic anhydrase 9 antibody at 1/1,000 dilution.)

Western Blot (WB) (Western Blot analysis of Carbonic anhydrase 9 on human lung lysates using anti- Carbonic anhydrase 9 antibody at 1/1,000 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti- Carbonic anhydrase 9 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti- Carbonic anhydrase 9 antibody. Counter stained with hematoxylin.)
Related Product Information for anti-CAIX antibody
Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes including respiration, calcification, acid-base balance and bone resorption, as well as the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. The human CA2 gene, which maps to chromosome 8q21, encodes CA II, a cytoplasmic protein that has the highest turnover rate and widest tissue distribution of any known human CA isozyme. The human CA4 gene, which maps to chromosome 17q23, encodes CA IV, a membrane-anchored isozyme that is expressed on the luminal surfaces of pulmonary capillaries and proximal renal tubules. The human CA9, CA12 and CA14 genes, which map to chromosomes 9p13, 15q22 and 1q21, respectively, encode transmembrane proteins that have unique patterns of tissue-specific expression. CA IX is specifically expressed in clear-cell renal carcinomas, whereas CA XII is highly expressed in normal tissues, such as kidney, colon and pancreas. Human CA XIV is also expressed in normal tissues, such as brain, but differs from CA XII in its expression pattern.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
768
UniProt Accession #
Molecular Weight
50kDa
NCBI Official Full Name
carbonic anhydrase IX
NCBI Official Synonym Full Names
carbonic anhydrase 9
NCBI Official Symbol
CA9
NCBI Official Synonym Symbols
MN; CAIX
NCBI Protein Information
carbonic anhydrase 9
UniProt Protein Name
Carbonic anhydrase 9
UniProt Gene Name
CA9
UniProt Synonym Gene Names
G250; MN; CA-IX; CAIX; RCC-associated antigen G250

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]

Uniprot Description

Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.

Research Articles on CAIX

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Product Notes

The CAIX ca9 (Catalog #AAA3012332) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CA IX Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CA IX can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1,000-1:2,000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the CAIX ca9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CA IX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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