Rabbit anti-Human C9 Polyclonal Antibody | anti-C9 antibody

C9, ID (C9, Complement component C9, Complement component C9a, Complement component C9b) (AP)

Cat. Num. AAA6280100

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: C9; Polyclonal Antibody; ID (C9; Complement component C9; Complement component C9a; Complement component C9b) (AP); Complement component C9b; anti-C9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human C9.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-C9 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 191-220 in the central region of human C9.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ZR-75-1 cell line lysates (35ug/lane) using 033068. This demonstrates that 033068 detected the C9 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in human liver lysate (20ug) using 033068 (1:1000). Secondary IgG (HRP) Goat Anti-Rabbit at 1:10,000. Predicted band size: 63kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Product Categories/Family for anti-C9 antibody

Antibodies; Complement

References

1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253 (2010). 2. Han, S., et al. Hum. Immunol. 71(7):727-730 (2010). 3. Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361 (2010). 4. Bunkenborg, J., et al. Proteomics 4(2):454-465 (2004). 5. Hofsteenge, J., et al. J. Biol. Chem. 274(46):32786-32794 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 4502511
• NCBI GeneID: 735
• NCBI Accession #: NP_001728.1
• NCBI GenBank Nucleotide #: NM_001737.3
• UniProt Accession #: P02748
• Molecular Weight: 63,173 Da
• NCBI Official Full Name: complement component C9
• NCBI Official Synonym Full Names: complement component 9
• NCBI Official Symbol: C9
• NCBI Protein Information: complement component C9
• UniProt Protein Name: Complement component C9
• Protein Family: Complement component
• UniProt Gene Name: C9
• UniProt Entry Name: CO9_HUMAN

NCBI Description

This gene encodes the final component of the complement system. It participates in the formation of the Membrane Attack Complex (MAC). The MAC assembles on bacterial membranes to form a pore, permitting disruption of bacterial membrane organization. Mutations in this gene cause component C9 deficiency. [provided by RefSeq, Feb 2009]

Uniprot Description

C9: Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC. Defects in C9 are a cause of complement component 9 deficiency (C9D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. Belongs to the complement C6/C7/C8/C9 family.

Protein type: Membrane protein, multi-pass; Extracellular matrix; Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 5p14-p12

Cellular Component: membrane attack complex; integral to plasma membrane; cytoplasm; extracellular region; plasma membrane

Biological Process: complement activation, alternative pathway; regulation of complement activation; innate immune response; hemolysis by symbiont of host red blood cells; complement activation, classical pathway

Disease: Macular Degeneration, Age-related, 15; Complement Component 9 Deficiency