Rabbit anti-Human C7 Polyclonal Antibody | anti-C7 antibody

C7, ID (C7, Complement component C7) (Azide free) (HRP)

Cat. Num. AAA6280038

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: C7; Polyclonal Antibody; ID (C7; Complement component C7) (Azide free) (HRP); Complement component C7; anti-C7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human C7.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-C7 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 375-403 in the central region of human C7, UniProt Accession #P10643.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of C7 in Ramos cell line lysates (35ug/lane) using 033038. C7 (arrow) was detected using 033038.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of C7 in Formalin-fixed paraffin-embedded human lymph tissue using 033038 followed by peroxidase-conjugated secondary antibody and DAB staining.)

Product Categories/Family for anti-C7 antibody

Antibodies; Complement

References

1. Davila, S., et.al., Genes Immun. 11 (3), 232-238 (2010). 2. Kuijpers, T.W., et.al., Mol. Immunol. 47 (4), 671-677 (2010). 3. Wheeler, H.E., et.al., PLoS Genet. 5 (10), E1000685 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 45580688
• NCBI GeneID: 730
• NCBI Accession #: NP_000578.2
• NCBI GenBank Nucleotide #: NM_000587.2
• UniProt Accession #: P10643; Q6P3T5; Q92489
• Molecular Weight: 93,518 Da
• NCBI Official Full Name: complement component C7
• NCBI Official Synonym Full Names: complement component 7
• NCBI Official Symbol: C7
• NCBI Protein Information: complement component C7
• UniProt Protein Name: Complement component C7
• Protein Family: Complement component
• UniProt Gene Name: C7
• UniProt Entry Name: CO7_HUMAN

NCBI Description

C7 is a component of the complement system. It participates in the formation of Membrane Attack Complex (MAC). People with C7 deficiency are prone to bacterial infection. [provided by RefSeq, Jul 2008]

Uniprot Description

C7: Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor. Defects in C7 are a cause of complement component 7 deficiency (C7D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. Belongs to the complement C6/C7/C8/C9 family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: membrane attack complex; extracellular region

Biological Process: cellular sodium ion homeostasis; cytolysis; complement activation, alternative pathway; regulation of complement activation; innate immune response; complement activation, classical pathway; complement activation

Disease: Complement Component 7 Deficiency