Mouse beta-Actin Monoclonal Antibody | anti-ACTB antibody

beta-Actin Mouse Monoclonal Antibody

Cat. Num. AAA9610326

• Gene Names: ACTB; BRWS1; PS1TP5BP1
• Reactivity: Human, Mouse, Rat, Pig, Zebrafish, Cow, Sheep, Rabbit, Goat, Guinea pig, Dog, Monkey, Hamster, Chicken, Plants, Rice, Fish
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: Affinity-Chromatography
• Synonyms: beta-Actin; Monoclonal Antibody; beta-Actin Mouse Monoclonal Antibody; ACTB; Actin; cytoplasmic 1; Beta-actin; Beta actin; BRWS1; beta; Beta cytoskeletal actin; PS1TP5-binding protein 1; PS1TP5BP1; anti-ACTB antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Pig, Zebrafish, Cow, Sheep, Rabbit, Goat, Guinea pig, Dog, Monkey, Hamster, Chicken, Plants, Rice, Fish
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: beta-Actin Mouse Monoclonal antibody detects endogenous levels of total beta-Actin protein.
• Purity/Purification: Affinity-Chromatography
• Form/Format: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 375

• Applicable Applications for anti-ACTB antibody: Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:3000-1:10000; IHC: 1:200; IF/ICC: 1:200
• Immunogen: Full-length beta-Actin protein of human
• Subcellular Location: Cytoplasm > Cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of various lysates, using beta-Actin Mouse Monoclonal Antibody.)

Related Product Information for anti-ACTB antibody

Description: Beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.
Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subunit Structure: Interacts with CPNE1 (via VWFA domain) and CPNE4 (via VWFA domain) (By similarity). Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCSAM. Interacts with TBC1D21 (By similarity). Interacts with DHX9 (via C-terminus); this interaction is direct and mediates the attachment to nuclear ribonucleoprotein complexes (PubMed:11687588).
Post-translational Modifications: ISGylated.Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity). Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).
Similarity: Belongs to the actin family.

References

Yi S, Yu M, Yang S, Miron RJ, Zhang Y; Journal: Stem Cells. Tcf12, A Member of Basic Helix-Loop-Helix Transcription Factors, Mediates Bone Marrow Mesenchymal Stem Cell Osteogenic Differentiation In Vitro and In Vivo. Shao Q, Zhang P, Ma Y, Lu Z, Meng J, Li H, Wang X, Chen D, Zhang M, Han Y, Liu H, Ma S; Journal: Gene. MicroRNA-139-5p affects cisplatin sensitivity in human nasopharyngeal carcinoma cells by regulating the epithelial-to-mesenchymal transition. Yang YF, Zhou YD, Hu JC, Luo FL, Xie Y, Shen YY, Bian WX, Yin ZN, Li HL, Zhang XL; Journal: Immunology. Ficolin-A/2, acting as a new regulator of macrophage polarization, mediates the inflammatory response in experimental mouse colitis. Liang X, Sun R, Zhao X, Zhang Y, Gu Q, Dong X, Zhang D, Sun J, Sun B; Journal: J Cell Mol Med. Rictor regulates the vasculogenic mimicry of melanoma via the AKT-MMP-2/9 pathway. Zhang W, Zhou P, Meng A, Zhang R, Zhou Y; Journal: J Cell Mol Med. Down-regulating Myoferlin inhibits the vasculogenic mimicry of melanoma via decreasing MMP-2 and inducing mesenchymal-to-epithelial transition.

NCBI and Uniprot Product Information

• NCBI GI #: 4501885
• NCBI GeneID: 60
• NCBI Accession #: NP_001092.1
• NCBI GenBank Nucleotide #: NM_001101.4
• UniProt Accession #: P60709; P02570; P70514; P99021; Q11211; Q64316; Q75MN2; Q96B34; Q96HG5
• Molecular Weight: Observed: 43 kD; Predicted: 42 kDa
• NCBI Official Full Name: actin, cytoplasmic 1
• NCBI Official Synonym Full Names: actin beta
• NCBI Official Symbol: ACTB
• NCBI Official Synonym Symbols: BRWS1; PS1TP5BP1
• NCBI Protein Information: actin, cytoplasmic 1
• UniProt Protein Name: Actin, cytoplasmic 1
• Protein Family: Beta-actin-like protein
• UniProt Gene Name: ACTB

NCBI Description

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017]

Uniprot Description

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947).