Rabbit C4BPB Polyclonal Antibody | anti-C4BPB antibody

C4BPB Polyclonal Antibody

Cat. Num. AAA3011522

• Gene Names: C4BPB; C4BP
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: C4BPB; Polyclonal Antibody; C4BPB Polyclonal Antibody; C4b-binding protein beta chain; anti-C4BPB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: C4BPB polyclonal antibody detects endogenous levels of C4BPB protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 252

• Applicable Applications for anti-C4BPB antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant full length Human C4BPB.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cells, using C4BPB antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach using C4BPB antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse kidney using C4BPB antibody at dilution of 1:100 (40x lens).)

Related Product Information for anti-C4BPB antibody

Complement component 4-binding protein (C4BP) is a plasma glycoprotein that inhibits the classical pathway of complement activation, which is mediated through antibody targeting of foreign antigen. Structurally, C4BP is a disulfide linked, multimeric protein that is composed of seven alpha chains and one beta chain. C4BP functions as a cofactor for C3beta inactivator in the cleavage of C3beta, and accelerates the decay of C4betaC2alpha (C3 convertase) by acting as a cofactor in the cleavage of C4beta by factor I. Streptococcal strains that express Ig-binding cell surface molecules, which are members of the M protein family, can bind to overlapping C4beta binding sites in C4BP and therefore, interfere with the classical pathway of complement activation. Bacteria-bound C4BP may be an evolved mechanism that downregulates complement activation in the bacterial host microenvironment, thereby reducing the occurances of bacterial opsonization and phagocytosis.

NCBI and Uniprot Product Information

• NCBI GI #: 4502505
• NCBI GeneID: 725
• NCBI Accession #: NP_000707.1
• NCBI GenBank Nucleotide #: NP_000707.1
• UniProt Accession #: P20851
• Molecular Weight: ~ 54kDa
• NCBI Official Full Name: C4b-binding protein beta chain isoform 1
• NCBI Official Synonym Full Names: complement component 4 binding protein beta
• NCBI Official Symbol: C4BPB
• NCBI Official Synonym Symbols: C4BP
• NCBI Protein Information: C4b-binding protein beta chain
• UniProt Protein Name: C4b-binding protein beta chain
• Protein Family: C4b-binding protein
• UniProt Gene Name: C4BPB
• UniProt Entry Name: C4BPB_HUMAN

NCBI Description

This gene encodes a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. A single, unique beta-chain encoded by this gene assembles with seven identical alpha-chains into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway. C4b-binding protein has a regulatory role in the coagulation system also, mediated through the beta-chain binding of protein S, a vitamin K-dependent protein that serves as a cofactor of activated protein C. The genes encoding both alpha and beta chains are located adjacent to each other on human chromosome 1 in the regulator of complement activation gene cluster. Alternative splicing gives rise to multiple transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

C4BPB: Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. It also interacts with anticoagulant protein S and with serum amyloid P component. The beta chain binds protein S. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular space; extracellular region; plasma membrane

Molecular Function: protein binding

Biological Process: negative regulation of complement activation, classical pathway; positive regulation of protein catabolic process; regulation of complement activation; innate immune response; blood coagulation; complement activation, classical pathway