Rabbit anti-Human C4B Polyclonal Antibody | anti-C4B antibody

C4B, ID (C4A, CO4, CPAMD2, Complement C4-A, Acidic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2, Complement C4 beta chain, Complement C4-A alpha chain, C4a anaphylatoxin, C4b-A, C4d-A, Complement C4 gamma chain) (FITC)

Cat. Num. AAA6279872

• Gene Names: C4B; CH; C4F; CO4; C4B1; C4B2; C4B3; C4B5; C4BD; C4B12; C4B_2; CPAMD3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: C4B; Polyclonal Antibody; ID (C4A; CO4; CPAMD2; Complement C4-A; Acidic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2; Complement C4 beta chain; Complement C4-A alpha chain; C4a anaphylatoxin; C4b-A; C4d-A; Complement C4 gamma chain) (FITC); C4A; Complement C4 gamma chain; anti-C4B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human C4B.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-C4B antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 655-684 in the central region of human C4B.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

((1:2000) + HepG2 whole cell lysate Lysates/proteins at 20ug/lane.Secondary IgG (H+L) (HRP) goat anti-rabbit 1:10,000 dilution. Predicted band size : 193kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-C4B antibody

This gene encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9.

Product Categories/Family for anti-C4B antibody

Antibodies; Complement

References

1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 2. Fernando, M.M., et al. Hum. Mutat. 31(7):866-874(2010). 3. Mostafa, G.A., et al. J. Neuroimmunol. 223 (1-2), 115-119 (2010). 4. Hamad, O.A., et al. J. Immunol. 184(5):2686-2692(2010). 5. Mougey, R. Immunohematology 26(1):30-38(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 178557739
• NCBI GeneID: 721
• NCBI Accession #: NP_001002029.3
• NCBI GenBank Nucleotide #: NM_001002029.3
• UniProt Accession #: P0C0L4; P0C0L5
• Molecular Weight: 192,751 Da
• NCBI Official Full Name: complement C4-B preproprotein
• NCBI Official Synonym Full Names: complement component 4B (Chido blood group)
• NCBI Official Symbol: C4B
• NCBI Official Synonym Symbols: CH; C4F; CO4; C4B1; C4B2; C4B3; C4B5; C4BD; C4B12; C4B_2; CPAMD3
• NCBI Protein Information: complement C4-B; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3; Chido form of C4; basic complement C4; complement C4B1a
• UniProt Protein Name: Complement C4-A
• Protein Family: Complement C4
• UniProt Gene Name: C4A
• UniProt Synonym Gene Names: CO4; CPAMD2
• UniProt Entry Name: CO4A_HUMAN

NCBI Description

This gene encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9. [provided by RefSeq, Jul 2008]

Uniprot Description

C4A: C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway. Defects in C4A are the cause of complement component 4A deficiency (C4AD). A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis. Defects in C4A are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Interindividual copy- number variation (CNV) of complement component C4 and associated polymorphisms result in different susceptibilities to SLE. The risk of SLE susceptibility has been shown to be significantly increased among subjects with only two copies of total C4. A high copy number is a protective factor against SLE.

Protein type: Secreted, signal peptide; Inhibitor; Secreted

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: plasma membrane; extracellular region

Molecular Function: complement component C1q binding; endopeptidase inhibitor activity

Biological Process: regulation of complement activation; innate immune response; inflammatory response; complement activation, classical pathway; complement activation

Disease: Complement Component 4a Deficiency; Blood Group, Chido/rodgers System