Rabbit C22orf32 Polyclonal Antibody | anti-SMDT1 antibody
C22orf32 Antibody
Gene Names
SMDT1; DDDD; EMRE; C22orf32; dJ186O1.1
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
C22orf32; Polyclonal Antibody; C22orf32 Antibody; Chromosome 22 open reading frame 32; CV032_HUMAN; DDDD; dJ186O1.1; mitochondrial; UPF0466 protein C22orf32; anti-SMDT1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
C22orf32 Antibody detects endogenous levels of total C22orf32.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-SMDT1 antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from Human MYOCD.
Conjugation
Unconjugated
Similarity
The GXXXX[G/A/S] motif at the C-terminal part of the transmembrane region mediates interaction with MCU and is required to activate the calcium-conducting pore in the uniporter complex. The poly-Asp region at the C-terminus mediates interaction with the polybasic region of MICU1. Belongs to the SMDT1/EMRE family.
Post Translational Modifications
Undergoes proteolytic degradation in neurons: degraded by AFG3L2 before SMDT1/EMRE assembly with the uniporter complex, limiting the availability of SMDT1/EMRE for MCU assembly and promoting efficient assembly of gatekeeper subunits with MCU.
Subunit Structure
Component of the uniplex complex, composed of MCU, MCUB, MICU1, MICU2 and EMRE/SMDT1. Interacts (via the transmembrane region) with MCU (via the first transmembrane region); the interaction is direct. Interacts (via the poly-Asp region) with MICU1 (via polybasic region); the interaction is direct. Interacts (via its C-terminal poly-Asp tail) with MCUR1; the interaction is direct. Unprocessed form interacts (via transit peptide) with MAIP1.
Function
Essential regulatory subunit of the mitochondrial calcium uniporter complex (uniplex), a complex that mediates calcium uptake into mitochondria. Required to bridge the calcium-sensing proteins MICU1 and MICU2 with the calcium-conducting subunit MCU. Plays a central role in regulating the uniplex complex response to intracellular calcium signaling. Acts by mediating activation of MCU and retention of MICU1 to the MCU pore, in order to ensure tight regulation of the uniplex complex and appropriate responses to intracellular calcium signaling.
Note
MAIP1 is required to assist sorting of EMRE/SMDT1 into mitochondrion by protecting EMRE/SMDT1 against protein degradation by YME1L1, thereby ensuring SMDT1/EMRE maturation by the mitochondrial processing peptidase (PMPCA and PMPCB) (PubMed:27642048).
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Product Categories/Family for anti-SMDT1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,441 Da
NCBI Official Full Name
essential MCU regulator, mitochondrial
NCBI Official Synonym Full Names
single-pass membrane protein with aspartate-rich tail 1
NCBI Official Symbol
SMDT1
NCBI Official Synonym Symbols
DDDD; EMRE; C22orf32; dJ186O1.1
NCBI Protein Information
essential MCU regulator, mitochondrial; UPF0466 protein C22orf32, mitochondrial; single-pass membrane protein with aspartate-rich tail 1, mitochondrial
UniProt Protein Name
Essential MCU regulator, mitochondrial
UniProt Gene Name
SMDT1
UniProt Synonym Gene Names
C22orf32; EMRE
UniProt Entry Name
EMRE_HUMAN
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Product Notes
The SMDT1 smdt1 (Catalog #AAA9629180) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The C22orf32 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's C22orf32 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the SMDT1 smdt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C22orf32, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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