Rabbit anti-Human C2/HCFC2 Polyclonal Antibody | anti-HCFC2 antibody

Anti-Host Cell Factor C2/HCFC2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101960

• Gene Names: HCFC2; HCF2; HCF-2
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: C2/HCFC2; Polyclonal Antibody; Anti-Host Cell Factor C2/HCFC2 Antibody; Rabbit Polyclonal; HCFC2 Antibody; Rabbit PAb; Antigen Affinity Purified; host cell factor C2; Anti-HCF-2 Antibody; Anti-HCF2 Antibody; anti-HCFC2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Host Cell Factor C2/HCFC2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-HCFC2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to the center region of the Human HCFC2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human HCFC2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-HCFC2 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:87 kDaObserved band size:80 kDa)

Related Product Information for anti-HCFC2 antibody

HCFC2 (Host Cell Factor C2) is a Protein Coding gene. The HCFC2 gene, located on 12q23.3, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, and frog. This gene encodes one of two proteins that interact with VP16, a herpes simplex virus protein that initiates virus infection. Both the encoded protein and the original Herpes host cell factor interact with VP16 through a beta-propeller domain. HCFC2 was necessary for the transcription of a large subset of other interferon regulatory factor 2 (IRF2)-dependent interferon-regulated genes. Deleterious mutations of Hcfc2 may therefore increase susceptibility to diverse infectious diseases. Diseases associated with HCFC2 include Herpes Simplex.

References

Yokoyama A, et al. (2004) Leukemia proto-oncoprotein mll forms a set1-like histone methyltransferase complex with menin to regulate hox gene expression. Mol Cell Biol 24 (13): 5639-5649.Shamay M, et al. (2012) A protein array screen for kaposi's sarcoma-associated herpesvirus lana interactors links lana to tip60, pp2a activity, and telomere shortening. J Virol 86 (9): 5179-5191.Gudkova D, et al. (2019) Hcf-2 inhibits cell proliferation and activates differentiation-gene expression programs. Nucleic Acids Res 47 (11): 5792-5808.

NCBI and Uniprot Product Information

• NCBI GI #: 7019405
• NCBI GeneID: 29915
• NCBI Accession #: NP_037452.1
• NCBI GenBank Nucleotide #: NM_013320.2
• UniProt Accession #: Q9Y5Z7; B2R8Q5
• Molecular Weight: 86,779 Da
• NCBI Official Full Name: host cell factor 2
• NCBI Official Synonym Full Names: host cell factor C2
• NCBI Official Symbol: HCFC2
• NCBI Official Synonym Symbols: HCF2; HCF-2
• NCBI Protein Information: host cell factor 2; C2 factor
• UniProt Protein Name: Host cell factor 2
• Protein Family: Host cell factor
• UniProt Gene Name: HCFC2
• UniProt Synonym Gene Names: HCF-2
• UniProt Entry Name: HCFC2_HUMAN

NCBI Description

This gene encodes one of two proteins which interact with VP16, a herpes simplex virus protein that initiates virus infection. Both the encoded protein and the original Herpes host cell factor interact with VP16 through a beta-propeller domain. The original Herpes host cell factor, however, is effective at initiating viral infection while the encoded protein is not. Transcripts of varying length due to alternative polyadenylation signals have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

HCFC2: Binds MLL. Component of the MLL1/MLL complex, at least composed of MLL, ASH2L, RBBP5, DPY30, WDR5, MEN1, HCFC1 and HCFC2.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 12q23.3

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; nucleus

Molecular Function: transcription coactivator activity

Biological Process: regulation of transcription from RNA polymerase II promoter; viral reproduction; negative regulation of transcription from RNA polymerase II promoter