Rabbit C2 Polyclonal Antibody | anti-C2 antibody

C2, NT (C2, Complement C2, C3/C5 convertase, Complement C2b fragment, Complement C2a fragment) (APC)

Cat. Num. AAA6279804

• Gene Names: C2; CO2; ARMD14
• Reactivity: Human, Hamster
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: C2; Polyclonal Antibody; NT (C2; Complement C2; C3/C5 convertase; Complement C2b fragment; Complement C2a fragment) (APC); Complement C2a fragment; anti-C2 antibody

• Host: Rabbit
• Reactivity: Human, Hamster
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human C2. Species Crossreactivity: hamster
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-C2 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 147-176 in the N-terminal region of human C2, UniProt Accession #NP_000054; P06681.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in CHO cell line lysates (35ug/lane) using 032881.This demonstrates the C2 antibody detected the C2 protein (arrow).)

Related Product Information for anti-C2 antibody

Component C2 is a serum glycoprotein that functions as part of the classical pathway of the complement system. Activated C1 cleaves C2 into C2a and C2b. The serine proteinase C2a then combines with complement factor 4b to create the C3 or C5 convertase. Deficiency of C2 has been reported to associated with certain autoimmune diseases and SNPs in this gene have been associated with altered susceptibility to age-related macular degeneration. This gene localizes within the class III region of the MHC on the short arm of chromosome 6. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described in publications but their full-length sequence has not been determined.

Product Categories/Family for anti-C2 antibody

Antibodies; Complement

References

1. Hu, M., et al. Pharmacogenet. Genomics 20(10):634-637(2010). 2. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 3. Liu, X., et al. Retina (Philadelphia, Pa.) 30(8):1177-1184(2010). 4. Han, S., et al. Hum. Immunol. 71(7):727-730(2010). 5. Ishii, Y., et al. J. Immunol. 151(1):170-174(1993).

NCBI and Uniprot Product Information

• NCBI GI #: 14550407
• NCBI GeneID: 717
• NCBI Accession #: NP_000054
• NCBI GenBank Nucleotide #: NM_000063
• UniProt Accession #: P06681
• Molecular Weight: 83,268 Da
• NCBI Official Full Name: complement C2 isoform 1 preproprotein
• NCBI Official Synonym Full Names: complement component 2
• NCBI Official Symbol: C2
• NCBI Official Synonym Symbols: CO2; ARMD14
• NCBI Protein Information: complement C2; C3/C5 convertase; complement component C2
• UniProt Protein Name: Complement C2
• Protein Family: Complement C2
• UniProt Gene Name: C2
• UniProt Entry Name: CO2_HUMAN

Uniprot Description

Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor C4b to generate the C3 or C5 convertase.