Rabbit C1QBP Polyclonal Antibody | anti-C1QBP antibody

C1QBP (Complement Component 1 Q Subcomponent-Binding Protein, p32, HABP1, gC1qR, GC1QBP, SF2p32, gC1Q-R) (FITC)

Cat. Num. AAA6409086

• Gene Names: C1QBP; p32; HABP1; gC1qR; GC1QBP; SF2p32; gC1Q-R
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: C1QBP; Polyclonal Antibody; C1QBP (Complement Component 1 Q Subcomponent-Binding Protein; p32; HABP1; gC1qR; GC1QBP; SF2p32; gC1Q-R) (FITC); anti-C1QBP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes C1QBP. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-C1QBP antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human C1QBP.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using C1QBP antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human esophagus using C1QBP antibody at dilution of 1:100 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat lung using C1QBP antibody at dilution of 1:100 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using C1QBP antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-C1QBP antibody

Antibodies; Complement

NCBI and Uniprot Product Information

• NCBI GI #: 4502491
• NCBI GeneID: 708
• NCBI Accession #: NP_001203.1
• NCBI GenBank Nucleotide #: NM_001212.3
• UniProt Accession #: Q07021; Q2HXR8; Q9NNY8
• Molecular Weight: 31,362 Da
• NCBI Official Full Name: complement component 1 Q subcomponent-binding protein, mitochondrial
• NCBI Official Synonym Full Names: complement component 1, q subcomponent binding protein
• NCBI Official Symbol: C1QBP
• NCBI Official Synonym Symbols: p32; HABP1; gC1qR; GC1QBP; SF2p32; gC1Q-R
• NCBI Protein Information: complement component 1 Q subcomponent-binding protein, mitochondrial; p33; glycoprotein gC1qBP; hyaluronan-binding protein 1; ASF/SF2-associated protein p32; mitochondrial matrix protein p32; C1q globular domain-binding protein; splicing factor SF2-associ
• UniProt Protein Name: Complement component 1 Q subcomponent-binding protein, mitochondrial
• Protein Family: Complement component 1 Q subcomponent-binding protein
• UniProt Gene Name: C1QBP
• UniProt Synonym Gene Names: GC1QBP; HABP1; SF2P32; C1qBP
• UniProt Entry Name: C1QBP_HUMAN

NCBI Description

The human complement subcomponent C1q associates with C1r and C1s in order to yield the first component of the serum complement system. The protein encoded by this gene is known to bind to the globular heads of C1q molecules and inhibit C1 activation. This protein has also been identified as the p32 subunit of pre-mRNA splicing factor SF2, as well as a hyaluronic acid-binding protein. [provided by RefSeq, Jul 2008]

Uniprot Description

C1QBP: a multifunctional and multicompartmental protein involved in inflammation and infection processes, ribosome biogenesis, regulation of apoptosis, transcriptional regulation and pre-mRNA splicing. Originally identified via its binding interactions with Splicing Factor (SF-2). Multiple, diverse binding partners of C1QBP were subsequently identified, including the globular heads of complement component C1q, hyaluronic acid, selected protein kinases, the tumor suppressor ARF, and multiple antigens of bacterial and viral origin. Overexpressed in a number of cancer cell types, and has been implicated in the Warburg effect, whereby cancer cells shift their metabolism from oxidative phosphorylation to glycolysis. Inhibits the Mitochondrial Permeability Transition (MPT) pore, possibly serving a protective function against damage from oxidative stress. Binding to C1q inhibits C1. In complex with cytokeratin-1/KRT1 is a high affinty receptor for kininogen-1/HMWK. The secreted form may enhance both extrinsic and intrinsic coagulation pathways. The cell surface form may require docking with transmembrane proteins for downstream signaling which might be specific for a cell-type or response. Belongs to the MAM33 family.

Protein type: Motility/polarity/chemotaxis; Receptor, misc.; Nucleolus; Mitochondrial

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: extracellular space; cell surface; mitochondrion; membrane; mitochondrial matrix; cytoplasm; plasma membrane; nucleolus; cytosol; nucleus

Molecular Function: mRNA binding; protein binding; adrenergic receptor binding; protein kinase C binding; complement component C1q binding; hyaluronic acid binding; kininogen binding; transcription factor binding; transcription corepressor activity

Biological Process: negative regulation of interleukin-12 production; phosphoinositide 3-kinase cascade; positive regulation of cell adhesion; viral reproduction; transcription, DNA-dependent; apoptosis; positive regulation of apoptosis; RNA splicing; negative regulation of defense response to virus; negative regulation of transcription from RNA polymerase II promoter; positive regulation of protein kinase B signaling cascade; negative regulation of nuclear mRNA splicing, via spliceosome; negative regulation of interferon-gamma production; regulation of complement activation; innate immune response; immune response; blood coagulation; mRNA processing; blood coagulation, intrinsic pathway; complement activation, classical pathway; mature ribosome assembly