Rabbit anti-Human C1q Polyclonal Antibody
Rabbit anti Human C1q
Reactivity
Human
Applications
Immunoprecipitation
Synonyms
C1q; Polyclonal Antibody; Rabbit anti Human C1q; anti-C1q antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Form/Format
Depilidated, heat inactivaded, lyophilized,stable whole antiserum.
Applicable Applications for anti-C1q antibody
Immunoprecipitation (IP)
Immunogen
C1 component of human complement is a complex of three glycoproteins; C1q,c1r and C1s,C1q has a molecular weight of 492,0000 and is present in plasma in an average concentration of 80ug/mL.It consists of a complex of 18 polypeptide chains of three different types, A,B and C; 6 of each typle.The subcomponent C1q is responsible for the binding of C1q to immunocomplexes an membranes via de Fc portion of the immunoglobulin. The subunit C1q is an isolated as a homogenous protein for use in antiserum production. Freund's complete adjuvant is used in the first step of the immunization procedure.
Adsorption
Immunoaffinity adsorbed using insolubilized antigens as required,to eliminate antibodies reacting with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorben protein or immune complexes in the antiserum.
Cross-Reactivity
The antiserum does not cross react with any other component of Human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Protein concentration
Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum.No foreigns proteins added.
Antibody titre
Precipitin titre 1:32 when tested against in agar-block immunodiffussion titration against anappropiate reference.
Intented use
In precipitatin techniques as immunoelectrophoresis and single or double radial immunodiffusion to identify the presence of C1q or C1q containing complexes in human plasma or other body fluis or to detemrine its concentration. THe presence of non precipitatin antibodies has not been assayed. This does not exclude the use of the antiserum in more sensitive non -precipitating antibody-binding techniques if proper controls are included.
Directions for use
The lyophilized antiserum is shipped at ambient temperature and may be stored at 4°C, prolonged storage at or below -20°C.Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water. Dilutins may be prepared by adding phosphate buffered saline (PBS,pH 7.2) Repeated thawing and freezing should be avoided If a slight precipitation occcurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum.
Diluted antiserum should be stored at +4°,not refrozen, and preferably used the same day.
Diluted antiserum should be stored at +4°,not refrozen, and preferably used the same day.
Packing
Vial with 1 ml lyophilized anitserum.
Preparation and Storage
Store lyophilized product at 4°C.
If reconstituted , storre at or below -20°C.
If reconstituted , storre at or below -20°C.
Related Product Information for anti-C1q antibody
Identity and Specificity: The reactivity of the antiserum is restricted to C1q. In immunoelectrophoresis and radial immunodiffusion(ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified C1q .Complexes containing C1q may also react with the antiserum. No reaction is obtained with C1r,C1s or any other plasma protein component or serum.
Intended Use: In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion to identify the presence of C1q or C1q containing complexes in human plasma or other body fluids or to determine its concentration. The presence of non precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in more sensitive nonprecipitating antibody-binding techniques if proper controls are included.
Intended Use: In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion to identify the presence of C1q or C1q containing complexes in human plasma or other body fluids or to determine its concentration. The presence of non precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in more sensitive nonprecipitating antibody-binding techniques if proper controls are included.
Product Categories/Family for anti-C1q antibody
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Product Notes
The C1q (Catalog #AAA573500) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Human C1q reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's C1q can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP). Researchers should empirically determine the suitability of the C1q for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C1q, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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