Rabbit anti-Human C1orf33/MRTO4 Polyclonal Antibody | anti-MRTO4 antibody

Anti-C1orf33/MRTO4 Antibody, Rabbit Polyclonal

Cat. Num. AAA8107451

• Gene Names: MRTO4; MRT4; C1orf33; dJ657E11.4
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: C1orf33/MRTO4; Polyclonal Antibody; Anti-C1orf33/MRTO4 Antibody; Rabbit Polyclonal; MRTO4/MRT4 Antibody; Rabbit PAb; Antigen Affinity Purified; MRT4 homolog; ribosome maturation factor; Anti-C1orf33 Antibody; Anti-dJ657E11.4 Antibody; Anti-MRT4 Antibody; anti-MRTO4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MRTO4/MRT4
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MRTO4 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:500-1:2000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human MRTO4/MRT4 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human MRTO4/MRT4 (rh MRTO4/MRT4). MRTO4/MRT4 specific IgG was purified by Human MRTO4/MRT4 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human MRTO4 in human placenta with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human MRTO4 in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunoprecipitation (IP)

(Mouse RTO4 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-Mouse RTO4 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse RTO4 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size: 28 kDa)

Western Blot (WB)

(Anti-MRTO4 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:28 kDaObserved band size:28 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-MRTO4 antibody

MRTO4 (MRT4 Homolog, Ribosome Maturation Factor) is a Protein Coding gene. MRTO4, also known as MRT4, belongs to the universal ribosomal protein uL10 family. This gene encodes a protein sharing a low level of sequence similarity with ribosomal protein P0. MRTO4 is a nuclear paralog of the ribosomal protein P0, it binds pre-60S subunits at an early stage of assembly in the nucleolus, and is replaced by P0 in cytoplasmic pre-60S subunits and mature 80S ribosomes. The precise function of MRTO4 is currently unknown. It appears to be involved in mRNA turnover and ribosome assembly. MRTO4 is widely expressed in the appendix, lymph node, and other tissues. Diseases associated with MRTO4 include Bacterial Conjunctivitis and Robinow Syndrome, Autosomal Dominant 1.

References

Zhao J. et al., 2011, J Proteomics. 75 (2): 588-602. Havugimana PC. et al., 2012, Cell. 150 (5): 1068-81. Wu Z. et al., 2012, PLoS One. 7 (8): e43997. Michalec B. et al., 2010, Int J Biochem Cell Biol. 42 (5): 736-48.

NCBI and Uniprot Product Information

• NCBI GI #: 118572609
• NCBI GeneID: 51154
• NCBI Accession #: NP_057267.2
• NCBI GenBank Nucleotide #: NM_016183
• UniProt Accession #: Q9UKD2; Q5TG55; Q96SS6; Q9BPV9; B3KNB3
• Molecular Weight: 27,560 Da
• NCBI Official Full Name: Homo sapiens mRNA turnover 4 homolog (S. cerevisiae) (MRTO4), mRNA
• NCBI Official Synonym Full Names: mRNA turnover 4 homolog (S. cerevisiae)
• NCBI Official Symbol: MRTO4
• NCBI Official Synonym Symbols: MRT4; C1orf33; dJ657E11.4
• NCBI Protein Information: mRNA turnover protein 4 homolog; 60S acidic ribosomal protein PO; MRT4, mRNA turnover 4, homolog; ribosome assembly factor MRTO4
• UniProt Protein Name: mRNA turnover protein 4 homolog
• Protein Family: mRNA turnover protein
• UniProt Gene Name: MRTO4
• UniProt Entry Name: MRT4_HUMAN

NCBI Description

This gene encodes a protein sharing a low level of sequence similarity with ribosomal protein P0. While the precise function of the encoded protein is currently unknown, it appears to be involved in mRNA turnover and ribosome assembly. [provided by RefSeq, Jul 2008]

Uniprot Description

MRTO4: Involved in mRNA turnover and ribosome assembly. Belongs to the ribosomal protein L10P family.

Protein type: Nucleolus; RNA-binding

Chromosomal Location of Human Ortholog: 1p36.13

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nucleolus; nucleus

Biological Process: ribosome biogenesis and assembly