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Rabbit anti-Human c-Met Polyclonal Antibody | anti-c-Met antibody

c-Met, CT (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR) (HRP)

Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified
Synonyms
c-Met; Polyclonal Antibody; CT (Hepatocyte Growth Factor Receptor; Scatter Factor Receptor; HGFR; SFR) (HRP); anti-c-Met antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human c-Met. Detects a 45kD protein on Western Blots of A431 cell lysate.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-c-Met antibody
Immunohistochemistry (IHC) Frozen/Paraffin, Western Blot (WB)
Application Notes
IHC-F/P: Frozen and formalin-fixed paraffin-embedded sections. Epitope retrieval required using citrate buffer, pH 6.0 is required. Enzyme digestion is not required.
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide derived from the human c-Met C-terminus sequence, conjugated to a carrier protein. Expected Staining Pattern: Cytoplasmic
Conjugate
HRP
Positive Control
Breast carcinoma
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Product Categories/Family for anti-c-Met antibody
References
1. To, C.T., Tsao, M.S., The roles of hepatocyte growth factor/scatter factor and met receptor in human cancers (Review). Oncol Rep 5(5): 1013-24 (1998). 2. Camp, R.L., Rimm, E.B., Rimm, D.L., Met expression is associated with poor outcome in patients with axillary lymph node negative breast carcinoma. Cancer 86(11): 2259-65 (1999). 3. Nakajima, M., et al., The prognostic significance of amplification and overexpression of c-met and c-erb B-2 in human gastric carcinomas. Cancer 85(9): 1894-902 (1999). 4. Wagatsuma, S., et al., Tumor angiogenesis, hepatocyte growth factor, and c-Met expression in endometrial carcinoma. Cancer 82(3): 520-30 (1998). 5. Elias, J.M., et al., Sensitivity and Detection Efficiency of the PAP, ABC and LAB Methods. Am. J. Clin. Pathol. 92: 62-67 (1989). 6. Shi, Z.R., Itzkowitz, S.H., Kim, Y.S., A Comparison of Three Immunoperoxidase Techniques for Antigen Detection in Colorectal Carcinoma Tissues. J. Histochemistry and Cytochemistry 36(3): 317-322 (1988). 7. Kiernan, J.A., Histological and Histochemical Methods: Theory and Practice. New York: Pergamon Press (1981). 8. Sheehan, D.C., Hrapchak, B.B., Theory and Practice of Histotechnology. St. Louis: C.V. Mosby Co. (1980). 9. Taylor, Cote, Immunomicroscopy: A Diagnostic Tool for the Surgical Pathologist. 2nd Ed. W.B. Saunders Co. Philadelphia, (1994). 10. Elias, J.M., et al., Special Report: Quality Control in immunohistochemistry. Am. J. Clin. Path. 92: 836 (1989). 11. O'Leary, T.J., et al., Quality assurance for immunocytochemistry: Proposed guideline MM4-P. National Committee for Clinical Laboratory Standards (NCCLS). Wayne, PA. 1-46. (1997). 12. College of American Pathologists (CAP) Certification Program for Immunohistochemistry. Northfield, IL. Http://www.cap.org (800) 323-4040. 13. Clinical Laboratory Improvement Amendments of 1988: Final Rule, 57 FR 7163 (February 28, 1992. ) 14. Koretzik, K., et al., Metachromasia of AEC and its precision in immunoperoxidase techniques. Histochemistry. 86: 471-478. (1987).

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Product Notes

The c-Met (Catalog #AAA6487837) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The c-Met, CT (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's c-Met can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Frozen/Paraffin, Western Blot (WB). IHC-F/P: Frozen and formalin-fixed paraffin-embedded sections. Epitope retrieval required using citrate buffer, pH 6.0 is required. Enzyme digestion is not required. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the c-Met for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-Met, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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