Rabbit anti-Human c-Jun Polyclonal Antibody | anti-C-JUN antibody

c-Jun, phosphorylated (Ser243)

Cat. Num. AAA625400

• Gene Names: C-JUN; Jun
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: c-Jun; Polyclonal Antibody; phosphorylated (Ser243); Anti -c-Jun; anti-C-JUN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of c-Jun only when phosphorylated at serine 243.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide and 50% glycerol.

• Applicable Applications for anti-C-JUN antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:500-1:1,000; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthesized phosphopeptide derived from human c-Jun around the phosphorylation site of serine 243 (P-L-SP-P-I)
• Preparation and Storage: For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MBS625400)

Western Blot (WB)

(Western Blot analysis of extracts from HeLa cells untreated or treated with UV using MBS625400)

Product Categories/Family for anti-C-JUN antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 1911701
• NCBI GeneID: 396913
• UniProt Accession #: P56432
• Molecular Weight: 35,629 Da
• NCBI Official Full Name: c-Jun
• NCBI Official Symbol: C-JUN
• NCBI Official Synonym Symbols: Jun
• NCBI Protein Information: transcription factor AP-1; AP1; activator protein 1; proto-oncogene c-jun; V-jun avian sarcoma virus 17 oncogene homolog
• UniProt Protein Name: Transcription factor AP-1
• Protein Family: C-Jun-amino-terminal kinase-interacting protein
• UniProt Gene Name: JUN
• UniProt Entry Name: JUN_PIG

Uniprot Description

Function: Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.

Subunit structure: Heterodimer with either FOS or BATF3 or ATF7

By similarity. The ATF7/JUN heterodimer is essential for ATF7 transactivation activity. Interacts with MYBBP1A, SP1, SPIB and TCF20. Interacts with COPS5; indirectly leading to its phosphorylation. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with HIVEP3. Component of the SMAD3/SMAD4/JUN/FOS/complex which forms at the AP1 promoter site. The SMAD3/SMAD4 heterodimer acts syngernistically with the JUN/FOS heterodimer to activate transcription in response to TGF-beta. Interacts (via its basic DNA binding and leucine zipper domains) with SMAD3 (via an N-terminal domain); the interaction is required for TGF-beta-mediated transactivation of the SMAD3/SMAD4/JUN/FOS/complex

By similarity. Interacts with RNF187

Subcellular location: Nucleus

Post-translational modification: Phosphorylation enhances the transcriptional activity. Phosphorylated by PRKDC

Sequence similarities: Belongs to the bZIP family. Jun subfamily.Contains 1 bZIP domain.