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Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~35.7 kDa c-Jun protein using Rabbit Anti-c-Jun Polyclonal Antibody (SPC-753). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-c-Jun Polyclonal Antibody (SPC-753) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~35.7 kDa.)

Rabbit anti-Human c-Jun Polyclonal Antibody | anti-c-Jun antibody

c-Jun Antibody: ATTO 594

Gene Names
JUN; AP1; p39; AP-1; c-Jun
Reactivity
Human
Applications
Western Blot
Purity
Peptide Affinity Purified
Synonyms
c-Jun; Polyclonal Antibody; c-Jun Antibody: ATTO 594; Rabbit Anti-Human c-Jun Polyclonal; AP 1 Antibody; Proto-oncogene c-Jun Antibody; cJun Antibody; Enhancer Binding Protein AP1 Antibody; p39 Antibody; JUN Antibody; JUN_HUMAN Antibody; V jun avian sarcoma virus 17 oncogene homolog Antibody; Jun Activation Domain Binding Protein Antibody; JUNC Antibody; Transcription Factor AP 1 Antibody; Oncogene JUN Antibody; V-jun avian sarcoma virus 17 oncogene homolog Antibody; Jun proto oncogene Antibody; Proto oncogene c jun Antibody; Transcription Factor AP1 Antibody; V jun sarcoma virus 17 oncogene homolog Antibody; Proto-oncogene c-jun Antibody; Proto oncogene cJun Antibody; V jun sarcoma virus 17 oncogene homolog (avian) Antibody; AP1 Antibody; Activator protein 1 Antibody; JUN protein Antibody; Jun oncogene Antibody; Transcription factor AP-1 Antibody; vJun Avian Sarcoma Virus 17 Oncogene Homolog Antibody; anti-c-Jun antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Detects 35.7 kDa.
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
331
Applicable Applications for anti-c-Jun antibody
Western Blot (WB)
Application Notes
WB: 1:1000
Target
c-Jun
Immunogen
Synthetic peptide from the mid-protein of Human c-Jun (aa. 90-190)
Immunogen Species
Human
Cellular Localization
Nucleus
Conjugate
ATTO 594
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~35.7 kDa c-Jun protein using Rabbit Anti-c-Jun Polyclonal Antibody (SPC-753). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-c-Jun Polyclonal Antibody (SPC-753) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~35.7 kDa.)

Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~35.7 kDa c-Jun protein using Rabbit Anti-c-Jun Polyclonal Antibody (SPC-753). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-c-Jun Polyclonal Antibody (SPC-753) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~35.7 kDa.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35,676 Da
NCBI Official Full Name
transcription factor AP-1
NCBI Official Synonym Full Names
Jun proto-oncogene, AP-1 transcription factor subunit
NCBI Official Symbol
JUN
NCBI Official Synonym Symbols
AP1; p39; AP-1; c-Jun
NCBI Protein Information
transcription factor AP-1
UniProt Protein Name
Transcription factor AP-1
UniProt Gene Name
JUN
UniProt Synonym Gene Names
AP1

NCBI Description

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).

Research Articles on c-Jun

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Product Notes

The c-Jun jun (Catalog #AAA8000989) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The c-Jun Antibody: ATTO 594 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's c-Jun can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000. Researchers should empirically determine the suitability of the c-Jun jun for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-Jun, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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