Western Blot (WB)
(BUB1A Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the BUB1A antibody detected the BUB1A protein (arrow).)
Rabbit anti-Human BUB1A Polyclonal Antibody | anti-BUB1A antibody
BUB1A, NT (BUB1, BUB1L, Mitotic checkpoint serine/threonine-protein kinase BUB1, BUB1A) (APC)
ELISA: 1:1,000
WB: 1:50-100
Applications are based on unconjugated antibody.
Western Blot (WB)
(BUB1A Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the BUB1A antibody detected the BUB1A protein (arrow).)
Western Blot (WB)
(BUB1A Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the BUB1A antibody detected the BUB1A protein (arrow).)
Flow Cytometry (FC/FACS)
(BUB1A Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(BUB1A Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
Uniprot Description
BUB1: a mitotic-checkpoint protein kinase of the BUB family. Autophosphorylated when the cells enters mitosis. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage. High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index. LOF mutations seen in two colon cancer cell lines exhibiting chromosomal instability, in several leukemias and lymphomas, and in a pancreatic cancer cell line. BUB1 functions in mitotic spindle assembly, and may cause chromosomal instability. In mouse BRCA2 knockouts, mutations in BUB1 and BUBR1 are frequently seen in the resulting tumors. BUB1 and BUBR1 are overexpressed, but not mutated, in many gastric cancers.
Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Cell cycle regulation; Other group; BUB family
Chromosomal Location of Human Ortholog: 2q14
Cellular Component: nucleoplasm; kinetochore; membrane; cytoplasm; cytosol; condensed nuclear chromosome, pericentric region
Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity
Biological Process: cell proliferation; mitosis; viral reproduction; apoptosis; cell division; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; regulation of chromosome segregation; regulation of sister chromatid cohesion; mitotic cell cycle; protein amino acid phosphorylation; chromosome segregation
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Product Notes
The BUB1A bub1 (Catalog #AAA6279320) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BUB1A, NT (BUB1, BUB1L, Mitotic checkpoint serine/threonine-protein kinase BUB1, BUB1A) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BUB1A can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB). FC/FACS: 1:10-50 ELISA: 1:1,000 WB: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the BUB1A bub1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BUB1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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