Rabbit anti-Human PYCARD / ASC Polyclonal Antibody | anti-PYCARD antibody

Rabbit Polyclonal (IgG) to Human PYCARD / ASC

Cat. Num. AAA243671

• Gene Names: PYCARD; ASC; TMS; TMS1; CARD5; TMS-1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein G Purified
• Synonyms: PYCARD / ASC; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human PYCARD / ASC; Anti-PYCARD / ASC Antibody (aa31-46) IHC-plus; PYCARD; CARD5; HASC; ASC; TMS; TMS-1; PYD and CARD domain containing; TMS1; Human PYCARD; anti-PYCARD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: A mixture of synthetic peptides corresponding to amino acid residues 31-46 and 94-110 of human TMS1.
• Purity/Purification: Protein G Purified
• Form/Format: 0.02% sodium azide.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 195

• Applicable Applications for anti-PYCARD antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (1 - 2 ug/ml)
• Target Species: Human
• Immunogen Description: A mixture of synthetic peptides corresponding to amino acid residues 31-46 and 94-110 of human TMS1.
• Immunogen Type: Synthetic peptide
• Immunogen: PYCARD / ASC antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 31-46 and 94-110 of human TMS1.
• Antigen Modification: aa31-46
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-PYCARD antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Western blot analysis for TMS1 using antibody at 2 ug/ml against 10 µg/lane of A549 cell lysate.)

Related Product Information for anti-PYCARD antibody

Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, BCL10, Apaf-1, ARC, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified in human and mouse and designated ASC and TMS1. Ectopic expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells.

Product Categories/Family for anti-PYCARD antibody

Family: Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 10835256
• NCBI GeneID: 29108
• NCBI Accession #: NP_037390.2
• NCBI GenBank Nucleotide #: NM_013258.4
• UniProt Accession #: Q9ULZ3; Q96D12; Q9BSZ5; Q9HBD0; Q9NXJ8
• Molecular Weight: 15,030 Da
• NCBI Official Full Name: apoptosis-associated speck-like protein containing a CARD isoform a
• NCBI Official Synonym Full Names: PYD and CARD domain containing
• NCBI Official Symbol: PYCARD
• NCBI Official Synonym Symbols: ASC; TMS; TMS1; CARD5; TMS-1
• NCBI Protein Information: apoptosis-associated speck-like protein containing a CARD; target of methylation-induced silencing 1; caspase recruitment domain-containing protein 5
• UniProt Protein Name: Apoptosis-associated speck-like protein containing a CARD
• Protein Family: Apoptosis-associated speck-like protein
• UniProt Gene Name: PYCARD
• UniProt Synonym Gene Names: ASC; CARD5; TMS1; hASC
• UniProt Entry Name: ASC_HUMAN

NCBI Description

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PYCARD: Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Forms complexes with other DAPIN domain-containing proteins. Interacts with CIAS1/PYPAF1 and PYDC1. Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T- cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Tumor suppressor

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: mitochondrion; cell soma; endoplasmic reticulum; cytoplasm; extracellular region; nucleolus; IkappaB kinase complex; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; protease binding; caspase activator activity; Pyrin domain binding

Biological Process: positive regulation of apoptosis; apoptosis; myeloid dendritic cell activation during immune response; regulation of protein stability; positive regulation of JNK cascade; positive regulation of caspase activity; positive regulation of interleukin-1 beta secretion; signal transduction; positive regulation of activated T cell proliferation; defense response to Gram-negative bacterium; activation of NF-kappaB transcription factor; tumor necrosis factor-mediated signaling pathway; positive regulation of phagocytosis; inflammatory response; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; defense response to virus; positive regulation of adaptive immune response; caspase activation; positive regulation of interleukin-6 production; interleukin-1 beta production; negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of tumor necrosis factor production; negative regulation of interferon-beta production; myeloid dendritic cell activation; positive regulation of interferon-gamma production; positive regulation of actin filament polymerization; inhibition of NF-kappaB transcription factor; regulation of inflammatory response; innate immune response; regulation of autophagy; positive regulation of transcription factor activity; positive regulation of T cell activation; positive regulation of defense response to virus by host; activation of innate immune response; positive regulation of antigen processing and presentation of peptide antigen via MHC class II