Rabbit anti-Human BTN3A1 Polyclonal Antibody | anti-BTN3A1 antibody

BTN3A1, CT (BTN3A1, BTF5, Butyrophilin subfamily 3 member A1, CD277) (APC)

Cat. Num. AAA6279265

• Gene Names: BTN3A1; BTF5; BT3.1; CD277; BTN3.1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BTN3A1; Polyclonal Antibody; CT (BTN3A1; BTF5; Butyrophilin subfamily 3 member A1; CD277) (APC); CD277; anti-BTN3A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-BTN3A1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: BTN3A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 344-374 amino acids from the C-terminal region of human BTN3A1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(BTN3A1 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the BTN3A1 antibody detected the BTN3A1 protein (arrow).)

Product Categories/Family for anti-BTN3A1 antibody

Antibodies; CD Markers

References

Smith, I.A., et al. J. Immunol. 184(7):3514-3525(2010). Shi, J., et al. Nature 460(7256):753-757(2009). Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006). Mungall, A.J., et al. Nature 425(6960):805-811(2003). Rhodes, D.A., et al. Genomics 71(3):351-362(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 222418660
• NCBI GeneID: 11119
• NCBI Accession #: NP_001138481.1; NP_919423.1; NP_001138480.1; NP_008979.3
• NCBI GenBank Nucleotide #: NM_001145009.1
• UniProt Accession #: O00481; Q0P515; Q147X5; Q53F15; Q99420; Q9HCY1; A2A278; A8K2C8; B4DIQ1; B4DRM2; E9PGB4; E9PHG8
• Molecular Weight: 52,019 Da
• NCBI Official Full Name: butyrophilin subfamily 3 member A1 isoform d
• NCBI Official Synonym Full Names: butyrophilin, subfamily 3, member A1
• NCBI Official Symbol: BTN3A1
• NCBI Official Synonym Symbols: BTF5; BT3.1; CD277; BTN3.1
• NCBI Protein Information: butyrophilin subfamily 3 member A1; dJ45P21.3 (butyrophilin, subfamily 3, member A1)
• UniProt Protein Name: Butyrophilin subfamily 3 member A1
• Protein Family: Butyrophilin subfamily
• UniProt Gene Name: BTN3A1
• UniProt Synonym Gene Names: BTF5

NCBI Description

The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene (MIM 601610) and the BTN2 (e.g., BTN2A1; MIM 613590) and BTN3 (e.g., BNT3A1) genes, which have undergone tandem duplication, resulting in 3 copies of each (summary by Smith et al., 2010 [PubMed 20208008]).[supplied by OMIM, Nov 2010]

Uniprot Description

Plays a role in T-cell activation and in the adaptive immune response. Regulates the proliferation of activated T-cells. Regulates the release of cytokines and IFNG by activated T-cells. Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.