Rabbit anti-Rat BTLA Polyclonal Antibody | anti-BTLA antibody

Anti-BTLA Antibody, Rabbit Polyclonal

Cat. Num. AAA8102962

• Reactivity: Rat
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: BTLA; Polyclonal Antibody; Anti-BTLA Antibody; Rabbit Polyclonal; BTLA/CD272 Antibody; Rabbit PAb; Antigen Affinity Purified; B and T lymphocyte associated; anti-BTLA antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rat BTLA/CD272
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-BTLA antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Rat BTLA/CD272
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Rat BTLA/CD272, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-BTLA rabbit polyclonal antibody at 1:500 dilutionLane A: Rat liver tissue lysateLane B: Rat spleen tissue lysateLane C: Rat kidney tissue lysateLysates/proteins at 30 ug per laneSecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:35 kDaObserved band size:55 kDa)

Related Product Information for anti-BTLA antibody

BTLA is a inhibitory molecule which belongs to the Ig superfamily. It down-modulates immune responses. As such, reagents that regulate the binding of BTLA to its ligand or alter BTLA signaling have significant therapeutic promise. BTLA is crucial to understand the mechanism(s) of action of these antibodies before attempting clinical applications. BTLA is not expressed by naive T cells, but it is induced during activation and remains expressed on T helper type 1 (T(H)1) but not T(H)2 cells. BTLA is a third inhibitory receptor on T lymphocytes with similarities to cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and programmed death 1 (PD-1).

Product Categories/Family for anti-BTLA antibody

Cancer Drug Targets ITIM/ITAM Immunoreceptors and Related Molecules

References

Fourcade J, et al. (2012) CD8(+) T cells specific for tumor antigens can be rendered dysfunctional by the tumor microenvironment through upregulation of the inhibitory receptors BTLA and PD-1. Cancer Res. 72(4):887-96.Kojima R, et al. (2011) Molecular basis for herpesvirus entry mediator recognition by the human immune inhibitory receptor CD160 and its relationship to the cosignaling molecules BTLA and LIGHT. J Mol Biol. 413(4):762-72.Oki M, et al. (2011) A functional polymorphism in B and T lymphocyte attenuator is associated with susceptibility to rheumatoid arthritis. Clin Dev Immunol. 305656.

NCBI and Uniprot Product Information

• NCBI GI #: 47155571
• NCBI GeneID: 407756
• NCBI Accession #: NP_998795.1
• NCBI GenBank Nucleotide #: NM_213630.1
• UniProt Accession #: Q6PNM1; Q569B2
• Molecular Weight: 34,476 Da
• NCBI Official Full Name: B- and T-lymphocyte attenuator
• NCBI Official Synonym Full Names: B and T lymphocyte associated
• NCBI Official Symbol: Btla
• NCBI Protein Information: B- and T-lymphocyte attenuator; B and T lymphocyte attenuator; B- and T-lymphocyte-associated protein
• UniProt Protein Name: B- and T-lymphocyte attenuator
• Protein Family: B- and T-lymphocyte attenuator
• UniProt Gene Name: Btla
• UniProt Entry Name: BTLA_RAT

Uniprot Description

Function: Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response