Western Blot (WB)
((1ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat BTLA Polyclonal Antibody | anti-BTLA antibody
Goat anti-BTLA Antibody
Gene Names
BTLA; BTLA1; CD272
Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
BTLA; Polyclonal Antibody; Goat anti-BTLA Antibody; B and T lymphocyte associated; BTLA1; CD272; FLJ16065; MGC129743; B and T lymphocyte attenuator; BTLA antibody; B and T lymphocyte associated antibody; BTLA1 antibody; CD272 antibody; FLJ16065 antibody; MGC129743 antibody; B and T lymphocyte attenuator antibody; anti-BTLA antibody
Host
Goat
Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
KEAPTEYASICVRS
Sequence Length
289
Applicable Applications for anti-BTLA antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: Approx.35kDa band observed in Mouse Spleen lysates (calculated MW of 32.8kDa according to Human NP_861445.3 and of 34.2kDa according to Mouse NP_001032808.2). Recommended concentration: 1-3ug/ml.
Western Blot: Approx.35kDa band observed in Mouse Spleen lysates (calculated MW of 32.8kDa according to Human NP_861445.3 and of 34.2kDa according to Mouse NP_001032808.2). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence KEAPTEYASICVRS, from the C Terminus of the protein sequence according to NP_861445.3; NP_001078826.1.
Epitope
C Terminus
Note
This antibody is expected to recognize both reported isoforms (NP_861445.3; NP_001078826.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((1ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,317 Da
NCBI Official Full Name
B- and T-lymphocyte attenuator isoform 1
NCBI Official Synonym Full Names
B and T lymphocyte associated
NCBI Official Symbol
BTLA
NCBI Official Synonym Symbols
BTLA1; CD272
NCBI Protein Information
B- and T-lymphocyte attenuator
UniProt Protein Name
B- and T-lymphocyte attenuator
Protein Family
UniProt Gene Name
BTLA
UniProt Entry Name
BTLA_HUMAN
NCBI Description
This gene encodes a member of the immunoglobulin superfamily. The encoded protein contains a single immunoglobulin (Ig) domain and is a receptor that relays inhibitory signals to suppress the immune response. Alternative splicing results in multiple transcript variants. Polymorphisms in this gene have been associated with an increased risk of rheumatoid arthritis. [provided by RefSeq, Aug 2011]
Uniprot Description
BTLA: Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 3q13.2
Cellular Component: integral to membrane; plasma membrane
Biological Process: adaptive immune response; T cell costimulation
Research Articles on BTLA
1. our study confirms that CD200/BTLA deletions are recurrent genetic lesions in the biology of BCP-ALL
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Product Notes
The BTLA btla (Catalog #AAA421917) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-BTLA Antibody reacts with Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BTLA can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx.35kDa band observed in Mouse Spleen lysates (calculated MW of 32.8kDa according to Human NP_861445.3 and of 34.2kDa according to Mouse NP_001032808.2). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the BTLA btla for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KEAPTEYASI CVRS. It is sometimes possible for the material contained within the vial of "BTLA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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