Rabbit anti-Human BTK Polyclonal Antibody | anti-BTK antibody

BTK, ID (Tyrosine-protein Kinase BTK, Bruton Tyrosine Kinase, Agammaglobulinaemia Tyrosine Kinase, ATK, B Cell Progenitor Kinase, AGMX1, ATK, BPK) (APC)

Cat. Num. AAA6466085

• Gene Names: BTK; AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, Immunohistochemistry
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: BTK; Polyclonal Antibody; ID (Tyrosine-protein Kinase BTK; Bruton Tyrosine Kinase; Agammaglobulinaemia Tyrosine Kinase; ATK; B Cell Progenitor Kinase; AGMX1; BPK) (APC); anti-BTK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BTK.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-BTK antibody: FLISA, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 209-239 in the central region of human BTK, UniProt Accession #Q06187.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot to detect BTK in Ramos cell lysate usingMBS630528.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue reacted with theMBS630528, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS630528 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of 293 cells usingMBS630528, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-BTK antibody

BTK plays a crucial role in B-cell ontogeny. This protein transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Defects in BTK are the cause of X-linked agammaglobulinemia type 1 (XLA). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis or fatal sepsis or meningitis within the first years of life.

Product Categories/Family for anti-BTK antibody

Antibodies; Protein Kinases

References

1. Marquez, J.A., et al., EMBO J. 22(18):4616-4624 (2003). 2. Jefferies, C.A., et al., J. Biol. Chem. 278(28):26258-26264 (2003). 3. Horwood, N.J., et al., J. Exp. Med. 197(12):1603-1611 (2003). 4. Goodman, P.A., et al., Leuk. Lymphoma 44(6):1011-1018 (2003). 5. Noordzij, J.G., et al., J. Clin. Immunol. 22(5):306-318 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 4557377
• NCBI GeneID: 695
• NCBI Accession #: NP_000052.1
• NCBI GenBank Nucleotide #: NM_000061.2
• UniProt Accession #: Q06187; Q32ML5
• Molecular Weight: 80kDa
• NCBI Official Full Name: tyrosine-protein kinase BTK
• NCBI Official Synonym Full Names: Bruton agammaglobulinemia tyrosine kinase
• NCBI Official Symbol: BTK
• NCBI Official Synonym Symbols: AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1
• NCBI Protein Information: tyrosine-protein kinase BTK; B-cell progenitor kinase; agammaglobulinaemia tyrosine kinase; tyrosine-protein kinase BTK isoform (lacking exon 14); dominant-negative kinase-deficient Brutons tyrosine kinase
• UniProt Protein Name: Tyrosine-protein kinase BTK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: BTK
• UniProt Synonym Gene Names: AGMX1; ATK; BPK; ATK; BPK
• UniProt Entry Name: BTK_HUMAN

NCBI Description

The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. [provided by RefSeq, Nov 2008]

Uniprot Description

Btk: a tyrosine kinase of the Tec family. Plays a crucial role in B-cell ontogeny. Defects cause X-linked agammaglobulinemia, an immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. Truncated splice forms found in childhood leukemias may underlie radiation resistance of tumors through inhibition of apoptosis.

Protein type: Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Kinase, protein; TK group; Tec family

Chromosomal Location of Human Ortholog: Xq21.33-q22

Cellular Component: extrinsic to internal side of plasma membrane; mast cell granule; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cytoplasmic vesicle; nucleus; cytosol; lipid raft

Molecular Function: identical protein binding; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; metal ion binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; adaptive immune response; transcription, DNA-dependent; B cell activation; cell maturation; calcium-mediated signaling; protein amino acid phosphorylation; T cell receptor signaling pathway; regulation of cell proliferation; toll-like receptor 2 signaling pathway; activation of NF-kappaB transcription factor; regulation of B cell cytokine production; MyD88-dependent toll-like receptor signaling pathway; regulation of B cell apoptosis; B cell receptor signaling pathway; histamine secretion by mast cell; toll-like receptor signaling pathway; innate immune response; positive regulation of B cell differentiation; mesoderm development; cell differentiation; toll-like receptor 4 signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Isolated Growth Hormone Deficiency, Type Iii; Agammaglobulinemia, X-linked