Rabbit anti-Human, Mouse BTF3 Polyclonal Antibody | anti-BTF3 antibody

BTF3, NT (BTF3, NACB, Transcription factor BTF3, RNA polymerase B transcription factor 3) (MaxLight 405)

Cat. Num. AAA6279170

• Gene Names: BTF3; NACB; BTF3a; BTF3b; BETA-NAC
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BTF3; Polyclonal Antibody; NT (BTF3; NACB; Transcription factor BTF3; RNA polymerase B transcription factor 3) (MaxLight 405); RNA polymerase B transcription factor 3; anti-BTF3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.
• Sequence Length: 162

• Applicable Applications for anti-BTF3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: BTF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human BTF3.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-BTF3 antibody

This gene encodes the basic transcription factor 3. This protein forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes.

Product Categories/Family for anti-BTF3 antibody

Antibodies; Transcription Factors

References

Green, C.D., et al. Mol. Cancer Res. 5(11):1191-1200(2007). Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Kusumawidjaja, G., et al. Cancer Biol. Ther. 6(3):367-376(2007). Freire, M.A. Gene 345(2):271-277(2005). Harrington, J.J., et al. Nat. Biotechnol. 19(5):440-445(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 20070130
• NCBI GeneID: 689
• NCBI Accession #: NP_001198.2; NP_001032726.1
• NCBI GenBank Nucleotide #: NP_001198.2
• UniProt Accession #: P20290
• NCBI Official Full Name: transcription factor BTF3 isoform B
• NCBI Official Synonym Full Names: basic transcription factor 3
• NCBI Official Symbol: BTF3
• NCBI Official Synonym Symbols: NACB; BTF3a; BTF3b; BETA-NAC
• NCBI Protein Information: transcription factor BTF3
• UniProt Protein Name: Transcription factor BTF3
• Protein Family: Basic transcription factor
• UniProt Gene Name: BTF3
• UniProt Synonym Gene Names: NACB; NAC-beta
• UniProt Entry Name: BTF3_HUMAN

NCBI Description

This gene encodes the basic transcription factor 3. This protein forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]

Uniprot Description

BTF3: a general transcription factor. BTF3 can form a stable complex with RNA polymerase II. An interaction partner of protein kinase CK2. Required for the initiation of transcription. Expressed at high levels in glioblastoma multiforme. Overexpressed in the microsatellite instability (MIN) phenotypes in colorectal cancer. Up-regulated strongly in the mammary gland during pregnancy, suggesting an involvement in alveolar growth. Two alternatively spliced isoforms have been described.

Protein type: Transcription factor; Transcription initiation complex

Chromosomal Location of Human Ortholog: 5q13.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: transcription from RNA polymerase II promoter; protein transport; regulation of transcription, DNA-dependent; in utero embryonic development