Goat Bruno-like 5 Polyclonal Antibody | anti-BRUNOL5 antibody

Goat anti-Bruno-like 5 Antibody

Cat. Num. AAA420771

• Gene Names: CELF5; CELF-5; BRUNOL5; BRUNOL-5
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: Bruno-like 5; Polyclonal Antibody; Goat anti-Bruno-like 5 Antibody; BRUNOL5; bruno-like 5; RNA binding protein (Drosophila); BRUNOL-5; CELF5; Bruno (Drosophila) -like 5; RNA binding protein; CUG-BP and ETR-3 like factor 5; RNA-binding protein BRUNOL-5; BRUNOL5 antibody; RNA binding protein (Drosophila) antibody; BRUNOL-5 antibody; CELF5 antibody; RNA binding protein antibody; CUG-BP and ETR-3 like factor 5 antibody; RNA-binding protein BRUNOL-5 antibody; anti-BRUNOL5 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: EPPGGQPDGMKD
• Sequence Length: 485

• Applicable Applications for anti-BRUNOL5 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 1000.; Western Blot: Approx 55kDa band observed in Human Heart lysates (calculated MW of 52.4kDa according to NP_068757.2). Recommended concentration: 1-3ug/ml.
• Immunogen: Peptide with sequence C-EPPGGQPDGMKD, from the internal region (near the N Terminus) of the protein sequence according to NP_068757.2.
• Epitope: Internal region (near the N Terminus)
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 24762232
• NCBI GeneID: 60680
• NCBI Accession #: NP_068757.2
• NCBI GenBank Nucleotide #: NM_021938.3
• Molecular Weight: 44,479 Da
• NCBI Official Full Name: CUGBP Elav-like family member 5 isoform 1
• NCBI Official Synonym Full Names: CUGBP, Elav-like family member 5
• NCBI Official Symbol: CELF5
• NCBI Official Synonym Symbols: CELF-5; BRUNOL5; BRUNOL-5
• NCBI Protein Information: CUGBP Elav-like family member 5
• UniProt Protein Name: CUGBP Elav-like family member 5
• UniProt Gene Name: CELF5
• UniProt Synonym Gene Names: BRUNOL5; CELF-5
• UniProt Entry Name: CELF5_HUMAN

NCBI Description

This gene encodes a member of the the CELF/BRUNOL protein family, which contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing and translation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

CELF5: RNA-binding protein implicated in the regulation of pre- mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Belongs to the CELF/BRUNOL family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: cytoplasm; nucleus

Molecular Function: nucleotide binding; RNA binding

Biological Process: mRNA processing