Goat BRSK2/STK29 Polyclonal Antibody | anti-BRSK2 antibody
Goat anti-BRSK2/STK29 Antibody
Gene Names
BRSK2; SAD1; SADA; STK29; PEN11B; C11orf7
Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
BRSK2/STK29; Polyclonal Antibody; Goat anti-BRSK2/STK29 Antibody; BRSK2; SAD1; STK29; PEN11B; C11orf7; BR serine/threonine kinase 2; serine/threonine kinase 29; homolog of SAD-1 (C. elegans); chromosome 11 open reading frame 7; chromsosome 11 open reading frame 7; FLJ41362; protein kinase SAD1B; serine/threonine kinase; BRSK2 antibody; SAD1 antibody; STK29 antibody; PEN11B antibody; C11orf7 antibody; BR serine/threonine kinase 2 antibody; serine/threonine kinase 29 antibody; homolog of SAD-1 (C. elegans) antibody; chromosome 11 open reading frame 7 antibody; chromsosome 11 open reading frame 7 antibody; FLJ41362 antibody; protein kinase SAD1B antibody; serine/threonine kinase SAD-A antibody; BRSK2 / STK29; anti-BRSK2 antibody
Host
Goat
Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
CPEVIRGEKYDGRKAD
Sequence Length
668
Applicable Applications for anti-BRSK2 antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Preliminary experiments gave bands at approx 50-55kDa and 15kDa in human brain, mouse brain and rat brain lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 74.7kDa according to NP_003948.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the bands).
Western Blot: Preliminary experiments gave bands at approx 50-55kDa and 15kDa in human brain, mouse brain and rat brain lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 74.7kDa according to NP_003948.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the bands).
Immunogen
Peptide with sequence CPEVIRGEKYDGRKAD, from the internal region of the protein sequence according to NP_003948.2.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68,740 Da
NCBI Official Full Name
serine/threonine-protein kinase BRSK2 isoform 2
NCBI Official Synonym Full Names
BR serine/threonine kinase 2
NCBI Official Symbol
BRSK2
NCBI Official Synonym Symbols
SAD1; SADA; STK29; PEN11B; C11orf7
NCBI Protein Information
serine/threonine-protein kinase BRSK2
UniProt Protein Name
Serine/threonine-protein kinase BRSK2
Protein Family
UniProt Gene Name
BRSK2
UniProt Synonym Gene Names
C11orf7; PEN11B; SADA; STK29; BR serine/threonine-protein kinase 2
UniProt Entry Name
BRSK2_HUMAN
Uniprot Description
BRSK2: a serine/threonine protein kinase of the CAMK group. Closely related to AMPK. Activated by the kinase LKB1. BRSK1 and BRSK2 are required for nueronal polarization. Expressed in the brain and at low levels in the testis. Four alternatively spliced isoforms have been described.
Protein type: EC 2.7.11.26; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, CAMK; CAMK group; CAMKL family; BRSK subfamily
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: centrosome; cytoplasm; endoplasmic reticulum; nucleus; perinuclear region of cytoplasm
Molecular Function: ATP binding; ATPase binding; magnesium ion binding; protein kinase binding; protein serine/threonine kinase activity; tau-protein kinase activity
Biological Process: actin cytoskeleton reorganization; axonogenesis; cell division; establishment of cell polarity; exocytosis; G2/M transition of mitotic cell cycle; mitosis; neuron differentiation; peptidyl-serine phosphorylation; protein amino acid phosphorylation; regulation of ATPase activity
Research Articles on BRSK2
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Product Notes
The BRSK2 brsk2 (Catalog #AAA420842) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-BRSK2/STK29 Antibody reacts with Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's BRSK2/STK29 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Preliminary experiments gave bands at approx 50-55kDa and 15kDa in human brain, mouse brain and rat brain lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 74.7kDa according to NP_003948.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the bands). Researchers should empirically determine the suitability of the BRSK2 brsk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CPEVIRGEKY DGRKAD. It is sometimes possible for the material contained within the vial of "BRSK2/STK29, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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