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Western Blot (WB) (Western Blot: Sample: Recombinant INHbA, Rat.)

Mouse anti-Rat Inhibin Beta A (INHbA) Monoclonal Antibody | anti-INHbA antibody

Monoclonal Antibody to Inhibin Beta A (INHbA)

Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Inhibin Beta A (INHbA); Monoclonal Antibody; Monoclonal Antibody to Inhibin Beta A (INHbA); anti-INHbA antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
C1
Specificity
The antibody is a mouse monoclonal antibody raised against INHbA. It has been selected for its ability to recognize INHbA in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-GKVNICC KKQFFVSFKD IGWNDWIIAP SGYHANYCEG ECPSHIAGTS GSSLSFHSTV INHYRMRGHS PFANLKSCCV PTKLRPMSML YYDDGQNIIK
Sequence Length
239
Applicable Applications for anti-INHbA antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant INHbA (Gly314~Ser424) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2048444
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant INHbA, Rat.)

Western Blot (WB) (Western Blot: Sample: Recombinant INHbA, Rat.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Liver Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Rat Liver Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Heart Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Rat Heart Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Testis Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Rat Testis Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Adrenal Gland Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Rat Adrenal Gland Tissue.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47,406 Da
NCBI Official Full Name
inhibin beta A chain preproprotein
NCBI Official Synonym Full Names
inhibin beta A subunit
NCBI Official Symbol
Inhba
NCBI Protein Information
inhibin beta A chain
UniProt Protein Name
Inhibin beta A chain
Protein Family
UniProt Gene Name
Inhba

NCBI Description

subunit which can homodimerize or heterodimerize with Inhbb to regulate FSH secretion [RGD, Feb 2006]

Uniprot Description

INHBA: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q12.1

Cellular Component: extracellular region; extracellular space; perinuclear region of cytoplasm

Molecular Function: cytokine activity; growth factor activity; hormone activity; identical protein binding; peptide hormone binding; protein heterodimerization activity; receptor binding; transforming growth factor beta receptor binding

Biological Process: activin receptor signaling pathway; cell cycle arrest; cell development; G1/S transition of mitotic cell cycle; hair follicle development; hemoglobin biosynthetic process; hemopoietic progenitor cell differentiation; male gonad development; mesoderm formation; mesodermal cell differentiation; negative regulation of cell cycle; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of hair follicle development; odontogenesis; ovarian follicle development; palate development; positive regulation of cellular protein metabolic process; positive regulation of erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; progesterone secretion; regulation of apoptosis; regulation of follicle-stimulating hormone secretion; regulation of MAPK cascade; regulation of transcription from RNA polymerase II promoter; response to drug; striatal medium spiny neuron differentiation

Research Articles on INHbA

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Product Notes

The INHbA inhba (Catalog #AAA2012487) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Inhibin Beta A (INHbA) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Inhibin Beta A (INHbA) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the INHbA inhba for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-GKVNIC C KKQFFVSFKD IGWNDWIIAP SGYHANYCEG ECPSHIAGTS GSSLSFHSTV INHYRMRGHS PFANLKSCCV PTKLRPMSML YYDDGQNIIK. It is sometimes possible for the material contained within the vial of "Inhibin Beta A (INHbA), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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