Rabbit anti-Human BRIP1 Polyclonal Antibody | anti-BRIP1 antibody

BRIP1 Antibody

Cat. Num. AAA7053221

• Gene Names: BRIP1; OF; BACH1; FANCJ
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: BRIP1; Polyclonal Antibody; BRIP1 Antibody; Fanconi anemia group J protein; Protein FACJ; ATP-dependent RNA helicase BRIP1; BRCA1-associated C-terminal helicase 1; BRCA1-interacting protein C-terminal helicase 1; BRCA1-interacting protein 1; BACH1; FANCJ; anti-BRIP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 1249

• Applicable Applications for anti-BRIP1 antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1:20-1:200; IF: 1:50-1:200
• Immunogen: Recombinant Human Fanconi anemia group J protein (66-243AA)
• Conjugation: Non-conjugated
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis tissue using MBS7053221 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of Hela cells using MBS7053221 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-BRIP1 antibody

DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1.

NCBI and Uniprot Product Information

• NCBI GI #: 301897118
• NCBI GeneID: 83990
• NCBI Accession #: NP_114432.2
• NCBI GenBank Nucleotide #: NM_032043.2
• UniProt Accession #: Q9BX63; Q3MJE2; Q8NCI5
• Molecular Weight: 112,476 Da
• NCBI Official Full Name: Fanconi anemia group J protein
• NCBI Official Synonym Full Names: BRCA1 interacting protein C-terminal helicase 1
• NCBI Official Symbol: BRIP1
• NCBI Official Synonym Symbols: OF; BACH1; FANCJ
• NCBI Protein Information: Fanconi anemia group J protein
• UniProt Protein Name: Fanconi anemia group J protein
• Protein Family: Fanconi anemia group J protein
• UniProt Gene Name: BRIP1
• UniProt Synonym Gene Names: BACH1; FANCJ; Protein FACJ; BRCA1-interacting protein 1

NCBI Description

The protein encoded by this gene is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). This gene may be a target of germline cancer-inducing mutations. [provided by RefSeq, Jul 2008]

Uniprot Description

BRIP1: a DNA-dependent ATPase and DNA helicase required for the maintenance of chromosomal stability. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1. Binds directly to the BRCT domains of BRCA1. Defects in BRIP1 cause of susceptibility to breast cancer and Fanconi anemia. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Belongs to the DEAD box helicase family, DEAH subfamily. Two alternatively spliced human isoforms have been described.

Protein type: EC 3.6.4.13; Helicase; Oncoprotein

Chromosomal Location of Human Ortholog: 17q23.2

Cellular Component: cytoplasm; nuclear membrane; nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: DNA replication; DNA synthesis during DNA repair; regulation of transcription from RNA polymerase II promoter; strand displacement

Disease: Breast Cancer; Fanconi Anemia, Complementation Group J; Tracheoesophageal Fistula With Or Without Esophageal Atresia