Western Blot (WB)
(staining (1.5ug/ml) of Hela lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat anti-Human BRF2 Polyclonal Antibody | anti-BRF2 antibody
BRF2 (Transcription Factor IIIB 50kD Subunit, TFIIIB50, hTFIIIB50, B-related Factor 2, BRF-2, hBRFU, BRFU, PRO1470)
Gene Names
BRF2; BRFU; TFIIIB50
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
BRF2; Polyclonal Antibody; BRF2 (Transcription Factor IIIB 50kD Subunit; TFIIIB50; hTFIIIB50; B-related Factor 2; BRF-2; hBRFU; BRFU; PRO1470); Anti -BRF2 (Transcription Factor IIIB 50kD Subunit; anti-BRF2 antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes human BRF2.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Applicable Applications for anti-BRF2 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:16,000
Western Blot: 1-3ug/ml, observed in Hela and human placenta lysates on ~50kD bands
Dilution: ELISA: 1:16,000
Western Blot: 1-3ug/ml, observed in Hela and human placenta lysates on ~50kD bands
Immunogen
Synthetic peptide corresponding to C-QAARQAATSVPNPP, from human BRF2, at C-terminal (NP_060780.2).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(staining (1.5ug/ml) of Hela lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(staining (1.5ug/ml) of Hela lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Related Product Information for anti-BRF2 antibody
BRF2 is one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. BRF2 is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein.
Product Categories/Family for anti-BRF2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
46,533 Da
NCBI Official Full Name
BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like, isoform CRA_a
NCBI Official Synonym Full Names
BRF2, RNA polymerase III transcription initiation factor 50 kDa subunit
NCBI Official Symbol
BRF2
NCBI Official Synonym Symbols
BRFU; TFIIIB50
NCBI Protein Information
transcription factor IIIB 50 kDa subunit; BRF-2; hBRFU; hTFIIIB50; B-related factor 2; transcription factor IIB- related factor, TFIIIB50; RNA polymerase III transcription initiation factor BRFU; BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like
UniProt Protein Name
Transcription factor IIIB 50 kDa subunit
Protein Family
UniProt Gene Name
BRF2
UniProt Synonym Gene Names
BRFU; TFIIIB50; hTFIIIB50; BRF-2
UniProt Entry Name
BRF2_HUMAN
NCBI Description
This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein. [provided by RefSeq, Jul 2008]
Uniprot Description
TFIIIB50: General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. Belongs to the TFIIB family.
Protein type: Transcription regulation
Chromosomal Location of Human Ortholog: 8p11.23
Cellular Component: nucleoplasm
Molecular Function: zinc ion binding
Biological Process: transcription from RNA polymerase III promoter; regulation of transcription, DNA-dependent; transcription initiation; gene expression
Research Articles on BRF2
Similar Products
Product Notes
The BRF2 brf2 (Catalog #AAA6003677) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The BRF2 (Transcription Factor IIIB 50kD Subunit, TFIIIB50, hTFIIIB50, B-related Factor 2, BRF-2, hBRFU, BRFU, PRO1470) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BRF2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:16,000 Western Blot: 1-3ug/ml, observed in Hela and human placenta lysates on ~50kD bands. Researchers should empirically determine the suitability of the BRF2 brf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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