Western Blot (WB)
(WB Suggested Anti-BRF1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Thymus)
Rabbit BRF1 Polyclonal Antibody | anti-BRF1 antibody
BRF1 antibody - C-terminal region
Gene Names
Brf1; GTF3B; TAF3C; TAFIII90; TFIIIB90; mTFIIIB90; 2510002F24Rik
Reactivity
Guinea Pig, Mouse, Rabbit, Rat
Applications
Western Blot
Purity
Affinity Purified
Synonyms
BRF1; Polyclonal Antibody; BRF1 antibody - C-terminal region; anti-BRF1 antibody
Host
Rabbit
Reactivity
Guinea Pig, Mouse, Rabbit, Rat
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequence
Synthetic peptide located within the following region: GSPPRDDSQPPERASTKKLSRRKRATTRNSADPGTSTGKRLRPLLSAQPA
Sequence Length
676
Applicable Applications for anti-BRF1 antibody
Western Blot (WB)
Homology
Guinea Pig: 92%; Mouse: 100%; Rabbit: 92%; Rat: 93%
Immunogen
The immunogen is a synthetic peptide directed towards the C terminal region of mouse BRF1
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(WB Suggested Anti-BRF1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Thymus)
Western Blot (WB)
(WB Suggested Anti-BRF1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Thymus)
Related Product Information for anti-BRF1 antibody
This is a rabbit polyclonal antibody against BRF1. It was validated on Western Blot using a cell lysate as a positive control.
Target Description: Brf1 is a general activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters
Target Description: Brf1 is a general activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters
Product Categories/Family for anti-BRF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
74kDa
NCBI Official Full Name
transcription factor IIIB 90 kDa subunit
NCBI Official Synonym Full Names
BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit
NCBI Official Symbol
Brf1
NCBI Official Synonym Symbols
GTF3B; TAF3C; TAFIII90; TFIIIB90; mTFIIIB90; 2510002F24Rik
NCBI Protein Information
transcription factor IIIB 90 kDa subunit
UniProt Protein Name
Transcription factor IIIB 90 kDa subunit
Protein Family
UniProt Gene Name
Brf1
UniProt Synonym Gene Names
TFIIIB90; mTFIIIB90; BRF-1
UniProt Entry Name
TF3B_MOUSE
Uniprot Description
GTF3B: General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter. Belongs to the TFIIB family. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription initiation complex
Cellular Component: nucleus; transcription factor TFIIIB complex
Molecular Function: zinc ion binding; TATA-binding protein binding; metal ion binding
Biological Process: transcription initiation from RNA polymerase III promoter; transcription, DNA-dependent; regulation of transcription, DNA-dependent; transcription initiation; positive regulation of transcription from RNA polymerase III promoter; regulation of mRNA stability
Research Articles on BRF1
Similar Products
Product Notes
The BRF1 brf1 (Catalog #AAA3205026) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BRF1 antibody - C-terminal region reacts with Guinea Pig, Mouse, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BRF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the BRF1 brf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: GSPPRDDSQP PERASTKKLS RRKRATTRNS ADPGTSTGKR LRPLLSAQPA. It is sometimes possible for the material contained within the vial of "BRF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
