Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BRE antibody at 1:200 dilution.)
Rabbit BRE Polyclonal Antibody | anti-BRE antibody
BRE Antibody
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
BRE; Polyclonal Antibody; BRE Antibody; Affinity Purified Rabbit Polyclonal BRE Antibody; Polyclonal BRE antibody; Anti-BRE antibody; BRCC4 antibody; BRCC45 antibody; BRCA1-A complex subunit BRE antibody; BRE antibody; brain and reproductive organ-expressed protein antibody; BRCA1/BRCA2-containing complex subunit 4 antibody; BRCA1/BRCA2-containing complex subunit 45 antibody; anti-BRE antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-BRE antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF: 1:100-1:500
ICC: 1:100-1:500
IHC-P: 1:100-1:500
WB: 1:500-1:3000
ICC: 1:100-1:500
IHC-P: 1:100-1:500
WB: 1:500-1:3000
Cross-Reactivity
Human, Mouse
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BRE antibody at 1:200 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BRE antibody at 1:200 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded Cal27 xenograft using BRE antibody at a dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded Cal27 xenograft using BRE antibody at a dilution of 1:100)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Raji) using a 10% SDS PAGE gel and BRE antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Raji) using a 10% SDS PAGE gel and BRE antibody at a dilution of 1:1000)
Related Product Information for anti-BRE antibody
Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks. The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as an adapter that bridges the interaction between MERIT40/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins.
Product Categories/Family for anti-BRE antibody
Similar Products
Product Notes
The BRE (Catalog #AAA5312343) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's BRE can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF: 1:100-1:500 ICC: 1:100-1:500 IHC-P: 1:100-1:500 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the BRE for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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