Rabbit BRCC45 Polyclonal Antibody | anti-BRE antibody

BRCC45 Antibody

Cat. Num. AAA150821

• Gene Names: BRE; BRCC4; BRCC45
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: BRCC45 Antibody is affinity chromatography purified via peptide column.
• Synonyms: BRCC45; Polyclonal Antibody; BRCC45 Antibody; BRCC4; BRCA1-A complex subunit BRE; Brain and reproductive organ-expressed protein; brain and reproductive organ-expressed (TNFRSF1A modulator); anti-BRE antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: BRCC45 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 415

• Applicable Applications for anti-BRE antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: BRCC45 antibody can be used for detection of BRCC45 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: BRCC45 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human BRCC45.
• Buffer: BRCC45 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: BRCC45 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of BRCC45 in HeLa cell lysate in (A) the absence and (B) presence of blocking peptide with BRCC45 antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of BRCC45 in human brain tissue with BRCC45 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of BRCC45 in human brain tissue with BRCC45 antibody at 20 μg/mL.)

Related Product Information for anti-BRE antibody

BRCC45 Antibody: BRCC45 was initially suggested to be a housekeeping protein that is highly expressed in brain and reproductive organs. Later experiments indicated BRCC45 forms a complex with the breast and ovarian predisposition proteins BRCA1 and BRCA2 as well as RAD51 and BRCC36. This complex has a ubiquitin E3 ligase activity and is thought to enhance cellular survival following DNA damage. BRCC45 has also been suggested to function as a death receptor-associated anti-apoptotic protein by inhibiting the BID-induced activation of the mitochondrial apoptotic pathway. Higher levels of BRCC45 were detected in the majority of hepatocellular carcinomas, suggesting that BRCC45 may promote tumorigenesis when overexpressed. At least three isoforms of BRCC45 are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 21361171
• NCBI GeneID: 9577
• NCBI Accession #: NP_004890
• NCBI GenBank Nucleotide #: NM_004899.4
• UniProt Accession #: Q9NXR7; Q13880; Q4ZFX8; Q53SD0; Q969X9; Q96P06; A8K4X1; D6W562; D6W563
• Molecular Weight: 42,697 Da
• NCBI Official Full Name: BRCA1-A complex subunit BRE isoform 1
• NCBI Official Synonym Full Names: brain and reproductive organ-expressed (TNFRSF1A modulator)
• NCBI Official Symbol: BRE
• NCBI Official Synonym Symbols: BRCC4; BRCC45
• NCBI Protein Information: BRCA1-A complex subunit BRE; BRCA1/BRCA2-containing complex subunit 45; BRCA1/BRCA2-containing complex, subunit 4; brain and reproductive organ-expressed protein
• UniProt Protein Name: BRCA1-A complex subunit BRE
• Protein Family: BRCA1-A complex
• UniProt Gene Name: BRE
• UniProt Entry Name: BRE_HUMAN

Uniprot Description

BRE: Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti- apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regulate TNF-alpha signaling through its interactions with TNFRSF1A; however these effects may be indirect. Belongs to the BRE family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; DNA repair, damage; Apoptosis; Cytokine

Chromosomal Location of Human Ortholog: 2p23.2

Cellular Component: cytoplasm; nuclear ubiquitin ligase complex; nucleus

Molecular Function: protein binding; peroxisome targeting sequence binding; polyubiquitin binding; tumor necrosis factor receptor binding

Biological Process: positive regulation of DNA repair; apoptosis; double-strand break repair; response to ionizing radiation; chromatin modification; signal transduction; G2/M transition DNA damage checkpoint; response to DNA damage stimulus