Rabbit anti-Human BRAP Polyclonal Antibody | anti-BRAP antibody

BRAP, NT (BRCA1 Associated Protein, BRCA1-associated Protein, BRAP2, Impedes Mitogenic Signal Propagation, IMP, RING Finger Protein 52, Renal Carcinoma Antigen NY-REN-63, RNF52)

Cat. Num. AAA6006877

• Gene Names: BRAP; IMP; BRAP2; RNF52
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: BRAP; Polyclonal Antibody; NT (BRCA1 Associated Protein; BRCA1-associated Protein; BRAP2; Impedes Mitogenic Signal Propagation; IMP; RING Finger Protein 52; Renal Carcinoma Antigen NY-REN-63; RNF52); Anti -BRAP; anti-BRAP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human BRAP.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-BRAP antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA: 1:1000; Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
• Immunogen: Synthetic peptide corresponding to aa27-39 of human BRAP at N-terminal (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Breast (formalin-fixed, paraffin-embedded) stained with BRAP antibody followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Product Categories/Family for anti-BRAP antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 188497705
• NCBI GeneID: 8315
• NCBI Accession #: NP_006759.3
• NCBI GenBank Nucleotide #: NM_006768.3
• UniProt Accession #: Q7Z569; O43238; O75341; B9EGS8
• Molecular Weight: 67,305 Da
• NCBI Official Full Name: BRCA1-associated protein
• NCBI Official Synonym Full Names: BRCA1 associated protein
• NCBI Official Symbol: BRAP
• NCBI Official Synonym Symbols: IMP; BRAP2; RNF52
• NCBI Protein Information: BRCA1-associated protein; RING finger protein 52; galectin-2-binding protein; renal carcinoma antigen NY-REN-63; impedes mitogenic signal propagation
• UniProt Protein Name: BRCA1-associated protein
• Protein Family: BRCA1-associated protein
• UniProt Gene Name: BRAP
• UniProt Synonym Gene Names: RNF52; IMP
• UniProt Entry Name: BRAP_HUMAN

NCBI Description

The protein encoded by this gene was identified by its ability to bind to the nuclear localization signal of BRCA1 and other proteins. It is a cytoplasmic protein which may regulate nuclear targeting by retaining proteins with a nuclear localization signal in the cytoplasm. [provided by RefSeq, Jul 2008]

Uniprot Description

BRAP: Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto- polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.

Protein type: Ubiquitin conjugating system; Ligase; EC 6.3.2.-; Ubiquitin ligase; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: nuclear membrane; cytoplasm; ubiquitin ligase complex

Molecular Function: protein binding; zinc ion binding; nuclear localization sequence binding; nucleotide binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: Ras protein signal transduction; MAPKKK cascade; protein ubiquitination; negative regulation of signal transduction