Rabbit BOB1 Polyclonal Antibody | anti-BOB1 antibody

BOB1 Antibody HRP Conjugated

Cat. Num. AAA9460545

• Gene Names: POU2AF1; BOB1; OBF1; OCAB; OBF-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by Protein A
• Synonyms: BOB1; Polyclonal Antibody; BOB1 Antibody HRP Conjugated; B cell Oct binding protein 1; B cell specic coactivator OBF 1; B cell specic coactivator OBF1; B-cell-specic coactivator OBF-1; BOB 1; BOB-1; OBF 1; OBF1; OBF1_HUMAN; OCA B; OCA-B; OCAB; OCT binding factor 1; OCT-binding factor 1; POU domain class 2 associating factor 1; POU domain class 2-associati; anti-BOB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A
• Form/Format: 10mM Tris Buffered Saline containing 1% BSA, 50% glycerol and 0.09% Gentamicin.
• Concentration: 1mgml (varies by lot)

• Applicable Applications for anti-BOB1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:100-1000; IHC: 1:100-500
• Conjugation: HRP
• Immunogen: KLH conjugated synthetic peptide derived from human BOB1
• Preparation and Storage: Store at 4 degree C for 12 months.

Related Product Information for anti-BOB1 antibody

POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. The POU dimer from the OCT1 gene formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1. Studies of tissue-specific expression of immunoglobulin promoters demonstrate the importance of an octamer, ATTTGCAT, and the proteins that bind to it. This is a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. Oct-1 encodes one protein, NF-A1, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, which is encoded by Oct-2. A novel protein designated Bob 1 (B cell Oct binding protein 1), alternatively called OBF-1, specifically interacts with Oct-1 and Oct-2, enhancing their transcriptional efficacy. Bob 1 is expressed at highest levels in spleen and peripheral blood leukocytes and represents an Oct co-factor capable of conferring cell-specific activation of Oct-1 and Oct-2. Although having no intrinsic capacity for DNA binding, Bob 1 associates tightly with the octamer motif in the presence of Oct-1 and or Oct-2. The gene which encodes Bob 1 maps to human chromosome 11q23.1.

Product Categories/Family for anti-BOB1 antibody

Conjugated Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 167900478
• NCBI GeneID: 5450
• NCBI Accession #: NP_006226.2
• NCBI GenBank Nucleotide #: NM_006235.2
• UniProt Accession #: Q16633; Q14983; B2R8Z9; AAB34937
• Molecular Weight: 27,436 Da
• NCBI Official Full Name: POU domain class 2-associating factor 1
• NCBI Official Synonym Full Names: POU class 2 associating factor 1
• NCBI Official Symbol: POU2AF1
• NCBI Official Synonym Symbols: BOB1; OBF1; OCAB; OBF-1
• NCBI Protein Information: POU domain class 2-associating factor 1; B-cell-specific coactivator OBF-1; BOB-1; OCA-B; OCT-binding factor 1; POU domain, class 2, associating factor 1
• UniProt Protein Name: POU domain class 2-associating factor 1
• Protein Family: Protein
• UniProt Gene Name: POU2AF1
• UniProt Synonym Gene Names: OBF1
• UniProt Entry Name: OBF1_HUMAN

Uniprot Description

POU2AF1: Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6. Belongs to the POU2AF1 family.

Protein type: Transcription, coactivator/corepressor; Oncoprotein

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: nucleus

Molecular Function: protein binding; DNA binding; transcription coactivator activity; transcription cofactor activity

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; humoral immune response