Rabbit anti-Human, Mouse BNIP3L Polyclonal Antibody | anti-BNIP3L antibody

BNIP3L, BH3 Domain (BCL2/Adenovirus E1B 19kD Protein-interacting Protein 3 Like, NIP3L, NIP3-like Protein X, CL2/Adenovirus E1B 19kD Protein-interacting Protein 3A, Adenovirus E1B19K-binding Protein B5, BNIP3A, BNIP3H, NIX) (PE)

Cat. Num. AAA6465482

• Gene Names: BNIP3L; NIX; BNIP3a
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: BNIP3L; Polyclonal Antibody; BH3 Domain (BCL2/Adenovirus E1B 19kD Protein-interacting Protein 3 Like; NIP3L; NIP3-like Protein X; CL2/Adenovirus E1B 19kD Protein-interacting Protein 3A; Adenovirus E1B19K-binding Protein B5; BNIP3A; BNIP3H; NIX) (PE); anti-BNIP3L antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BNIP3L. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-BNIP3L antibody: Immunohistochemistry (IHC), FLISA
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected within aa100-150 (BH3 domain) of human BNIP3L (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-BNIP3L antibody

Antibodies; Apoptosis

References

1. Aerbajinai, W., et al., Blood 102(2):712-717 (2003). 2. Passer, B.J., et al., Proc. Natl. Acad. Sci. USA 100(5):2284-2289 (2003). 3. Ohi, N., et al., Cell Death Differ. 6(4):314-325 (1999). 4. Chen, G., et al., J. Biol. Chem. 274(1):7-10 (1999). 5. Yasuda, M., et al., Cancer Res. 59(3):533-537 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 4757860
• NCBI GeneID: 665
• NCBI Accession #: NP_004322
• NCBI GenBank Nucleotide #: NM_004331
• UniProt Accession #: O60238; Q5JW63; Q8NF87; B0AZS9
• Molecular Weight: 19,559 Da
• NCBI Official Full Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
• NCBI Official Synonym Full Names: BCL2/adenovirus E1B 19kDa interacting protein 3-like
• NCBI Official Symbol: BNIP3L
• NCBI Official Synonym Symbols: NIX; BNIP3a
• NCBI Protein Information: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A; BCL2/adenovirus E1B 19-kd protein-interacting protein 3a; NIP3-like protein X; NIP3L; adenovirus E1B19k-binding protein B5
• UniProt Protein Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
• Protein Family: BCL2/adenovirus E1B 19 kDa protein-interacting protein
• UniProt Gene Name: BNIP3L
• UniProt Synonym Gene Names: BNIP3A; BNIP3H; NIX; NIP3L
• UniProt Entry Name: BNI3L_HUMAN

Uniprot Description

BNIP3L: Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. May function as a tumor suppressor. Self-associates. Interacts with BNIP3 and STEAP3. Interacts with SPATA18/MIEAP. Interacts with human adenovirus-2 E1B 19 kDa protein. Belongs to the NIP3 family.

Protein type: Apoptosis; Membrane protein, integral; Mitochondrial; Endoplasmic reticulum; Nuclear envelope

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; integral to membrane; nuclear envelope; intrinsic to membrane

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; lamin binding

Biological Process: viral reproduction; positive regulation of apoptosis; defense response to virus; negative regulation of apoptosis