Rabbit anti-Human BNIP2 Polyclonal Antibody | anti-BNIP2 antibody

BNIP2, CT (BNIP2, NIP2, BCL2/adenovirus E1B 19kD protein-interacting protein 2) (FITC)

Cat. Num. AAA6278706

• Gene Names: BNIP2; NIP2; BNIP-2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BNIP2; Polyclonal Antibody; CT (BNIP2; NIP2; BCL2/adenovirus E1B 19kD protein-interacting protein 2) (FITC); BCL2/adenovirus E1B 19kD protein-interacting protein 2; anti-BNIP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-BNIP2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: BNIP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 405-435 amino acids from the C-terminal region of human BNIP2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(BNIP2 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the BNIP2 antibody detected the BNIP2 protein (arrow).)

Immunohistochemistry (IHC)

(BNIP2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BNIP2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-BNIP2 antibody

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. Though the specific function is unknown, it interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector.

Product Categories/Family for anti-BNIP2 antibody

Antibodies; Infectious Disease Adenovirus

References

Scott, G.B., et al. Biochem. J. 431(3):423-431(2010). Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). Valencia, C.A., et al. Biochem. Biophys. Res. Commun. 364(3):495-501(2007). Zhou, Y.T., et al. Exp. Cell Res. 303(2):263-274(2005). Shang, X., et al. J. Biol. Chem. 278(46):45903-45914(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 168480080
• NCBI GeneID: 663
• NCBI Accession #: NP_004321.2
• NCBI GenBank Nucleotide #: NM_004330.2
• UniProt Accession #: Q12982; B4DS94
• Molecular Weight: 36,018 Da
• NCBI Official Full Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 2
• NCBI Official Synonym Full Names: BCL2/adenovirus E1B 19kDa interacting protein 2
• NCBI Official Symbol: BNIP2
• NCBI Official Synonym Symbols: NIP2; BNIP-2
• NCBI Protein Information: BCL2/adenovirus E1B 19 kDa protein-interacting protein 2
• UniProt Protein Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 2
• Protein Family: BCL2/adenovirus E1B 19 kDa protein-interacting protein
• UniProt Gene Name: BNIP2
• UniProt Synonym Gene Names: NIP2
• UniProt Entry Name: BNIP2_HUMAN

NCBI Description

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. [provided by RefSeq, Mar 2011]

Uniprot Description

BNIP2: Implicated in the suppression of cell death. Interacts with the BCL-2 and adenovirus E1B 19 kDa proteins. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; nuclear envelope; cytosol

Molecular Function: identical protein binding; protein binding; calcium ion binding; GTPase activator activity

Biological Process: muscle cell differentiation; apoptosis; positive regulation of muscle cell differentiation; positive regulation of GTPase activity; negative regulation of apoptosis