Rabbit BMP2 Polyclonal Antibody | anti-BMP2 antibody

Anti-BMP2 Antibody (C-Terminus) IHC-plus

Cat. Num. AAA249851

• Gene Names: BMP2; BDA2; BMP2A
• Reactivity: Chicken, Zebrafish, Pig, Rabbit, Mouse, Sheep, Bovine, Rat, Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity purified
• Synonyms: BMP2; Polyclonal Antibody; Anti-BMP2 Antibody (C-Terminus) IHC-plus; Rabbit Polyclonal to Human BMP2; Human BMP2; BDA2; BMP-2; BMP-2A; BMP2A; Bone morphogenetic protein 2; Bone morphogenetic protein 2A; anti-BMP2 antibody

• Host: Rabbit
• Reactivity: Chicken, Zebrafish, Pig, Rabbit, Mouse, Sheep, Bovine, Rat, Human
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of BMP2 protein.
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS, pH 7.3, 0.01% sodium azide, 30% glycerol.
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 396

• Applicable Applications for anti-BMP2 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P (1:100); WB (1:500 - 1:1000); Optimal dilution to be determined by the researcher.
• Immunogen: BMP2 antibody was raised against kLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human BMP2.
• Immunogen Description: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human BMP2.
• Immunogen Type: Synthetic peptide - KLH conjugated
• Epitope: C-Terminus
• Target Species: Human
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry - Paraffin (IHC)

(Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot analysis of BMP2 expression in HepG2 (A); HeLa (B) whole cell lysates.)

Related Product Information for anti-BMP2 antibody

BMP2 Antibody, BDA2 Antibody, BMP-2 Antibody, BMP-2A Antibody, BMP2A Antibody, Bone morphogenetic protein 2 Antibody, Bone morphogenetic protein 2A Antibody Description: BMP2 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone morphogenic protein induces bone formation. BMP2 is a candidate gene for the autosomal dominant disease of fibrodysplasia (myositis) ossificans progressiva.

NCBI and Uniprot Product Information

• NCBI GI #: 4557369
• NCBI GeneID: 650
• NCBI Accession #: NP_001191.1
• NCBI GenBank Nucleotide #: NM_001200.3
• UniProt Accession #: P12643
• Molecular Weight: 44,702 Da
• NCBI Official Full Name: bone morphogenetic protein 2 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 2
• NCBI Official Symbol: BMP2
• NCBI Official Synonym Symbols: BDA2; BMP2A
• UniProt Protein Name: Bone morphogenetic protein 2
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP2
• UniProt Synonym Gene Names: BMP2A; BMP-2; BMP-2A
• UniProt Entry Name: BMP2_HUMAN

NCBI Description

This gene encodes a member of the transforming growth factor-beta (TGFB) superfamily. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which induces bone and cartilage formation. [provided by RefSeq, Nov 2015]

Uniprot Description

Induces cartilage and bone formation (PubMed:3201241). Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4 (PubMed:24362451).