Rabbit anti-Human Bmp10 Polyclonal Antibody | anti-Bmp10 antibody

Bmp10, NT (Bone Morphogenetic Protein 10, BMP-10) (Azide free) (HRP)

Cat. Num. AAA6465598

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: Bmp10; Polyclonal Antibody; NT (Bone Morphogenetic Protein 10; BMP-10) (Azide free) (HRP); anti-Bmp10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Bmp10.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Bmp10 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 7-35 in the N-terminal region of human Bmp10.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293 cell line lysates (35ug/lane) usingMBS625635. Bmp10(arrow) was detected usingMBS625635.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS625635, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS625635 for immunohistochemistry. BC = breast carcinoma)

Product Categories/Family for anti-Bmp10 antibody

Antibodies; Growth Factors; Cytokines

References

1. Neuhaus, H., et al., Mech. Dev. 80(2):181-184 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 7656928
• NCBI GeneID: 27302
• NCBI Accession #: NP_055297.1
• NCBI GenBank Nucleotide #: NM_014482.1
• UniProt Accession #: O95393; Q53R17; Q6NTE0
• Molecular Weight: 48,047 Da
• NCBI Official Full Name: bone morphogenetic protein 10 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 10
• NCBI Official Symbol: BMP10
• NCBI Protein Information: bone morphogenetic protein 10
• UniProt Protein Name: Bone morphogenetic protein 10
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP10
• UniProt Synonym Gene Names: BMP-10
• UniProt Entry Name: BMP10_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TGF-beta family of growth factors. Data suggest that the similar protein in mouse plays an important role in trabeculation of the embryonic heart. In human, this protein may signal through receptor serine/threonine kinases. [provided by RefSeq, Jul 2008]

Uniprot Description

BMP10: Required for maintaining the proliferative activity of embryonic cardiomyocytes by preventing premature activation of the negative cell cycle regulator CDKN1C/p57KIP and maintaining the required expression levels of cardiogenic factors such as MEF2C and NKX2-5. Acts as a ligand for ACVRL1/ALK1, BMPR1A/ALK3 and BMPR1B/ALK6, leading to activation of SMAD1, SMAD5 and SMAD8 transcription factors. Inhibits endothelial cell migration and growth. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2p13.3

Cellular Component: extracellular space; cell surface; cytoplasm; extracellular region; Z disc

Molecular Function: receptor serine/threonine kinase binding; protein binding; growth factor activity; telethonin binding; hormone activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: adult heart development; extracellular matrix organization and biogenesis; atrial cardiac muscle morphogenesis; positive regulation of transcription, DNA-dependent; ventricular cardiac muscle cell development; activin receptor signaling pathway; cardiac muscle cell proliferation; sarcomere organization; regulation of apoptosis; BMP signaling pathway; regulation of MAPKKK cascade; ventricular cardiac muscle morphogenesis; negative regulation of cell growth; cell adhesion; negative regulation of cell migration; positive regulation of cardiac muscle cell proliferation