Western Blot (WB)
(All lanes : Anti-BMI1 Antibody (C-term) at 1:1000 dilutionLane 1: A549 whole cell lysatesLane 2: Hela whole cell lysatesLane 3: MCF-7 whole cell lysatesLane 4: NIH/3T3 whole cell lysatesLysates/proteins at 20 ?g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 37 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human, Mouse BMI1 Polyclonal Antibody | anti-BMI1 antibody
BMI1 Antibody (C-term)
IF: 1:25
Western Blot (WB)
(All lanes : Anti-BMI1 Antibody (C-term) at 1:1000 dilutionLane 1: A549 whole cell lysatesLane 2: Hela whole cell lysatesLane 3: MCF-7 whole cell lysatesLane 4: NIH/3T3 whole cell lysatesLysates/proteins at 20 ?g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 37 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-BMI1 Antibody (C-term) at 1:1000 dilutionLane 1: A549 whole cell lysatesLane 2: Hela whole cell lysatesLane 3: MCF-7 whole cell lysatesLane 4: NIH/3T3 whole cell lysatesLysates/proteins at 20 ?g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 37 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunofluorescence (IF)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human cervical epithelial adenocarcinoma cell line) cells labeling BMI1 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing nuclear staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor 555 conjugated with Phalloidin (OB16636430) at 1/100 dilution (red).)
Immunofluorescence (IF)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human cervical epithelial adenocarcinoma cell line) cells labeling BMI1 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing nuclear staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor 555 conjugated with Phalloidin (OB16636430) at 1/100 dilution (red).)
Background: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. [provided by RefSeq, Sep 2015]
Uniprot Description
BMI1: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. Component of a PRC1-like complex. Interacts with RING1 and RING2. Interacts vwith CBX7 and CBX8. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1.
Protein type: Motility/polarity/chemotaxis; Oncoprotein; Ligase; EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 10p11.23
Cellular Component: nucleoplasm; nuclear body; heterochromatin; cytoplasm; PcG protein complex; nucleus; ubiquitin ligase complex
Molecular Function: protein binding; zinc ion binding; sequence-specific DNA binding; ubiquitin-protein ligase activity; chromatin binding
Biological Process: transcription, DNA-dependent; in utero embryonic development; positive regulation of immature T cell proliferation in the thymus; negative regulation of transcription from RNA polymerase II promoter; humoral immune response; embryonic skeletal morphogenesis; segment specification; rostrocaudal neural tube patterning; positive regulation of fibroblast proliferation; regulation of gene expression; positive regulation of ubiquitin-protein ligase activity; histone ubiquitination; DNA methylation; positive regulation of B cell proliferation; somatic stem cell division; hemopoiesis; brain development; histone acetylation
Research Articles on BMI1
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Product Notes
The BMI1 bmi1 (Catalog #AAA9232118) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BMI1 Antibody (C-term) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BMI1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ELISA( EIA). WB: 1:1000 IF: 1:25. Researchers should empirically determine the suitability of the BMI1 bmi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BMI1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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