Rabbit anti-Human BLNK Polyclonal Antibody | anti-BLNK antibody

Anti-BLNK Antibody, Rabbit Polyclonal

Cat. Num. AAA8106579

• Gene Names: BLNK; bca; AGM4; BASH; LY57; SLP65; BLNK-S; SLP-65
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: BLNK; Polyclonal Antibody; Anti-BLNK Antibody; Rabbit Polyclonal; BLNK/Ly-57/SLP-65 Antibody; Rabbit PAb; Antigen Affinity Purified; B-cell linker; Anti-AGM4 Antibody; Anti-BASH Antibody; Anti-bca Antibody; Anti-BLNK-S Antibody; Anti-LY57 Antibody; Anti-SLP-65 Antibody; Anti-SLP65 Antibody; anti-BLNK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human BLNK/Ly-57/SLP-65
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-BLNK antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000; IHC-P: 1:2000-1:10,000
• Immunogen: Recombinant Human BLNK/Ly-57/SLP-65 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human BLNK/Ly-57/SLP-65 (rh BLNK/Ly-57/SLP-65). BLNK/Ly-57/SLP-65 specific IgG was purified by Human BLNK/Ly-57/SLP-65 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human BLNK in human tonsil with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human BLNK in human spleen with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human BLNK in human brain with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)

Western Blot (WB)

(Anti-BLNK rabbit polyclonal antibody at 1:500 dilutionLane A: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:50 kDaObserved band size:70 kDa)

Related Product Information for anti-BLNK antibody

B-cell linker protein, also known as B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, Cytoplasmic adapter protein, Src homology 2 domain-containing leukocyte protein of 65 kDa, SLP-65 and BLNK, is a cytoplasm and cell membrane protein which contains oneSH2 domain. BLNK is expressed in B-cell lineage and fibroblast cell lines. Highest levels of expression is in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon. BLNK functions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways, regulating biological outcomes of B-cell function and development. BLNK plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. BLNK modulates AP1 activation. It is important for the activation of NF-kappa-B and NFAT. BLNK plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca2+mobilization and is required for trafficking of the BCR to late endosomes. BLNK may be required for the RAC1-JNK pathway. It plays a critical role in orchestrating the pro-B cell to pre-B cell transition. BLNK also plays an important role in BCR-induced B-cell apoptosis.Defects in BLNK are the cause of agammaglobulinemia type 4 (AGM4) which is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development.

Product Categories/Family for anti-BLNK antibody

Adaptor Proteins

References

Fu C., et al., 1998, Immunity 9:93-103. Minegishi Y., et al., 1999, Science 286:1954-1957. Chiu C.W., et al., 2002, EMBO J. 21:6461-6472. Taguchi T., et al., 2004, Immunology 112:575-582.

NCBI and Uniprot Product Information

• NCBI GI #: 17511914
• NCBI GeneID: 29760
• Molecular Weight: 44,451 Da
• NCBI Official Full Name: B-cell linker
• NCBI Official Synonym Full Names: B-cell linker
• NCBI Official Symbol: BLNK
• NCBI Official Synonym Symbols: bca; AGM4; BASH; LY57; SLP65; BLNK-S; SLP-65
• NCBI Protein Information: B-cell linker protein
• Protein Family: B-cell linker protein

NCBI Description

This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012]