BLNK active protein

BLNK Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121214

• Gene Names: BLNK; bca; AGM4; BASH; LY57; SLP65; BLNK-S; SLP-65
• Purity: >90% as determined by SDS-PAGE
• Synonyms: BLNK; BLNK Protein; Human; Recombinant (His Tag); Human BLNK/Ly-57/SLP-65 Protein (His Tag); AGM4 Protein; BASH Protein; bca Protein; BLNK-S Protein; LY57 Protein; SLP-65 Protein; SLP65 Protein; B-cell linker; BLNK active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser456

• Species: Human
• Activity: Measured by its ability to bind human BTK in a functional ELISA.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human BLNK isoform 1 (AAH18906.1)(Met 1-Ser 456) was fused with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for BLNK active protein

Background: B-cell linker protein, also known as B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, Cytoplasmic adapter protein, Src homology 2 domain-containing leukocyte protein of 65kDa, SLP-65 and BLNK, is a cytoplasm and cell membrane protein which contains oneSH2 domain. BLNK is expressed in B-cell lineage and fibroblast cell lines. Highest levels of expression is in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon. BLNK functions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways, regulating biological outcomes of B-cell function and development. BLNK plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. BLNK modulates AP1 activation. It is important for the activation of NF-kappa-B and NFAT. BLNK plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca2+mobilization and is required for trafficking of the BCR to late endosomes. BLNK may be required for the RAC1-JNK pathway. It plays a critical role in orchestrating the pro-B cell to pre-B cell transition. BLNK also plays an important role in BCR-induced B-cell apoptosis.Defects in BLNK are the cause of agammaglobulinemia type 4 (AGM4) which is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development.

Product Categories/Family for BLNK active protein

Adaptor Proteins

References

Fu C., et al., 1998, Immunity 9:93-103. Minegishi Y., et al., 1999, Science 286:1954-1957. Chiu C.W., et al., 2002, EMBO J. 21:6461-6472. Taguchi T., et al., 2004, Immunology 112:575-582.

NCBI and Uniprot Product Information

• NCBI GI #: 17511914
• NCBI GeneID: 29760
• Molecular Weight: 44,451 Da
• NCBI Official Full Name: B-cell linker
• NCBI Official Synonym Full Names: B-cell linker
• NCBI Official Symbol: BLNK
• NCBI Official Synonym Symbols: bca; AGM4; BASH; LY57; SLP65; BLNK-S; SLP-65
• NCBI Protein Information: B-cell linker protein
• Protein Family: B-cell linker protein

NCBI Description

This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012]