Immunohistochemistry (IHC)
(Human Testis (formalin-fixed, paraffin-embedded) stained with PTRH2 antibody at 5ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)
Rabbit anti-Human Bit1 Polyclonal Antibody
Bit1, NT (Peptidyl-tRNA Hydrolase 2, Mitochondrial, PTH 2, Bcl-2 Inhibitor of Transcription 1, PTRH2, PTH2, CGI-147)
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Bit1; Polyclonal Antibody; NT (Peptidyl-tRNA Hydrolase 2; Mitochondrial; PTH 2; Bcl-2 Inhibitor of Transcription 1; PTRH2; PTH2; CGI-147); Anti -Bit1; anti-Bit1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes human Bit1. Species sequence homology: Mouse and rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.02% sodium azide.
Applicable Applications for anti-Bit1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Dilution: Western Blot: 1-4ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Dilution: Western Blot: 1-4ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Immunogen
Synthetic peptide corresponding to 15aa from human Bit1 at N-terminal (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Human Testis (formalin-fixed, paraffin-embedded) stained with PTRH2 antibody at 5ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)
Immunohistochemistry (IHC)
(Human Testis (formalin-fixed, paraffin-embedded) stained with PTRH2 antibody at 5ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)
Related Product Information for anti-Bit1 antibody
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process. Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE. Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the a5b1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the a5b1 integrin.
Product Categories/Family for anti-Bit1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
Bcl-2 inhibitor of transcription
Uniprot Description
The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.
Similar Products
Product Notes
The Bit1 (Catalog #AAA641671) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Bit1, NT (Peptidyl-tRNA Hydrolase 2, Mitochondrial, PTH 2, Bcl-2 Inhibitor of Transcription 1, PTRH2, PTH2, CGI-147) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Bit1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Dilution: Western Blot: 1-4ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml. Researchers should empirically determine the suitability of the Bit1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bit1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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