SDS-Page
(W estern Blotting Experiment Lysates prepared from Hek293T cells transfected with rat WT BimEL alone (1, 4), cotransfected with rat WT BimEL and activated MEKK1 (MEKK1Δ) (2, 5) or with mutant S65A BimEL and MEKK1Δ (3, 6), were resolved by SDSPAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with 3% Milk-TBST buffer for one hour at room temperature, and incubated with Bim Pan antibody (1, 2, 3) or BimEL [pS65] (4, 5, 6) for two hours at room temperature in 3% Milk-TBST buffer. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using standard methods. The data show that the signal is detected only in lysates co-expressing rat WT BimEL and active MEKK1 (MEKK1Δ). This signal is abolished in cells expressing mutant rat BimEL S65A and MEKK1, verifying that the signal is site-phosphospecific.)
Rabbit anti-Human, Rat BimEL Polyclonal Antibody | anti-BCL2L11 antibody
BimEL, phosphorylated (Ser65) (Bcl2 interacting mediator of cell death)
Serum.
Serum.
Dilution: Western Blot: 1:1000
SDS-Page
(W estern Blotting Experiment Lysates prepared from Hek293T cells transfected with rat WT BimEL alone (1, 4), cotransfected with rat WT BimEL and activated MEKK1 (MEKK1Δ) (2, 5) or with mutant S65A BimEL and MEKK1Δ (3, 6), were resolved by SDSPAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with 3% Milk-TBST buffer for one hour at room temperature, and incubated with Bim Pan antibody (1, 2, 3) or BimEL [pS65] (4, 5, 6) for two hours at room temperature in 3% Milk-TBST buffer. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using standard methods. The data show that the signal is detected only in lysates co-expressing rat WT BimEL and active MEKK1 (MEKK1Δ). This signal is abolished in cells expressing mutant rat BimEL S65A and MEKK1, verifying that the signal is site-phosphospecific.)
SDS-Page
(W estern Blotting Experiment Lysates prepared from Hek293T cells transfected with rat WT BimEL alone (1, 4), cotransfected with rat WT BimEL and activated MEKK1 (MEKK1Δ) (2, 5) or with mutant S65A BimEL and MEKK1Δ (3, 6), were resolved by SDSPAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with 3% Milk-TBST buffer for one hour at room temperature, and incubated with Bim Pan antibody (1, 2, 3) or BimEL [pS65] (4, 5, 6) for two hours at room temperature in 3% Milk-TBST buffer. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using standard methods. The data show that the signal is detected only in lysates co-expressing rat WT BimEL and active MEKK1 (MEKK1Δ). This signal is abolished in cells expressing mutant rat BimEL S65A and MEKK1, verifying that the signal is site-phosphospecific.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2013]
Uniprot Description
BIM: a pro-apoptotic member of the BCL-2 protein family. Interacts with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and act as an apoptotic activator. Its expression can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role in neuronal and lymphocyte apoptosis. Transgenic studies in the mouse suggested that this protein functions as an essential initiator of the apoptosis in thymocyte-negative selection. Nineteen alternatively spliced transcript variants of this gene have been reported.
Protein type: Apoptosis
Chromosomal Location of Human Ortholog: 2q13
Cellular Component: microtubule; mitochondrial outer membrane; extrinsic to membrane; endomembrane system; cytosol
Molecular Function: protein binding; microtubule binding
Biological Process: nerve growth factor receptor signaling pathway; positive regulation of apoptosis; apoptosis; cell-matrix adhesion; pigmentation during development; myeloid cell homeostasis; B cell apoptosis; ear development; positive regulation of caspase activity; cellular process regulating host cell cycle in response to virus; positive regulation of apoptosis by virus; mammary gland development; B cell homeostasis; positive regulation of neuron apoptosis; T cell homeostasis; kidney development; post-embryonic organ morphogenesis; spleen development; caspase activation; thymus development; positive regulation of protein homooligomerization; in utero embryonic development; male gonad development; positive regulation of cell cycle; regulation of organ growth; lumen formation; odontogenesis of dentine-containing teeth; DNA damage response, signal transduction resulting in induction of apoptosis; regulation of pigmentation during development; spermatogenesis; brain development
Research Articles on BCL2L11
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Product Notes
The BCL2L11 bcl2l11 (Catalog #AAA614320) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BimEL, phosphorylated (Ser65) (Bcl2 interacting mediator of cell death) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BimEL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the BCL2L11 bcl2l11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BimEL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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