Goat Bif-1 Polyclonal Antibody | anti-SH3GLB1 antibody

Bif-1 (Bax interacting factor-1, SH3GLB1; Bif-1; CGI-61; KIAA0491; SH3-domain GRB2-like endophilin B1; endophilin B1, SH3-containing protein SH3GLB1; SH3-domain, GRB2-like, endophilin B1; SH3-containing protein SH3GLB1 CGI-61 protein endophilin B1)

Cat. Num. AAA615026

• Gene Names: SH3GLB1; Bif-1; CGI-61; KIAA0491; dJ612B15.2
• Reactivity: Canine, Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Bif-1; Polyclonal Antibody; Bif-1 (Bax interacting factor-1; SH3GLB1; CGI-61; KIAA0491; SH3-domain GRB2-like endophilin B1; endophilin B1; SH3-containing protein SH3GLB1; SH3-domain; GRB2-like; SH3-containing protein SH3GLB1 CGI-61 protein endophilin B1); Anti -Bif-1 (Bax interacting factor-1; anti-SH3GLB1 antibody

• Host: Goat
• Reactivity: Canine, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes Human, Mouse, Rat, canine Bif-1 (Bax interacting factor-1, SH3GLB1)
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-SH3GLB1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 38kD band observed in lysates of Human T cell line Jurkat and in Human Lung and Placenta (calculated MW of 40.8kD according to NP_057093.1). Recommended concentration: 0.3-1.0 ug/ml. A minor band of unknown identity was also consistently observed at 50kD in Jurkat and Placenta. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field-have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?, Staining (0.3ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Optimal dilutions to be determined by the researcher.
• Immunogen: C-QKGKVPITYLELLN, C Terminus
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-SH3GLB1 antibody

The Bcl-2 family consists of anti-apoptotic (or cell survival) genes, such as Bcl-2, Bcl-XL and pro-apoptotic (or cell death) genes including Bax and BAD. In addition to its anti-apoptotic effect, Bcl-2 also inhibits progression through the cell cycle. Functional interactions between family members as well as binding to other cellular proteins modulate their activities. Bcl-2 family members are important in normal tissue development, homeostasis, and disease states. Bcl-2 prevents cell death by forming heterodimers with Bax and Bad (1). Bax promotes cell death by forming homodimers, which can be blocked by forming heterodimers with Bcl-2 or BCL-XL. Recently, using yeast two-hybrid system Cuddeback et al (2) have identified a cDNA encoding a 365 amino acid protein, which interacts physically with Bax protein and influences cell life and death. Bif-1 contains a Src homology 3 (SH3) domain in the C-terminus. It does not contain any of the conserved Bcl-2 homology (BH) domains of the Bcl-2 family proteins. Human Bif-1 protein shares 96% and 42% identity at the amino acids level with mouse and C. elegans, respectively. Overexpression of Bif-1 in transfected cell lines promotes Bax conformational change, caspase activation, and apoptotic cell death following growth factor withdrawal. Bif-1 RNA is abundantly expressed in heart, skeletal muscle, kidney, and placenta.

Product Categories/Family for anti-SH3GLB1 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 13469879
• NCBI GeneID: 51100
• UniProt Accession #: Q9Y371; Q5H8U5; Q9H3Z0; Q9NR47; Q9NYA9
• Molecular Weight: 40,796 Da
• NCBI Official Full Name: Bax-interacting factor 1
• NCBI Official Synonym Full Names: SH3-domain GRB2-like endophilin B1
• NCBI Official Symbol: SH3GLB1
• NCBI Official Synonym Symbols: Bif-1; CGI-61; KIAA0491; dJ612B15.2
• NCBI Protein Information: endophilin-B1; endophilin B1; OTTHUMP00000011912; OTTHUMP00000011913; Bax-interacting factor 1; SH3-containing protein SH3GLB1; SH3 domain-containing GRB2-like protein B1
• UniProt Protein Name: Endophilin-B1
• Protein Family: Endophilin
• UniProt Gene Name: SH3GLB1
• UniProt Synonym Gene Names: KIAA0491
• UniProt Entry Name: SHLB1_HUMAN

NCBI Description

This gene encodes a SRC homology 3 domain-containing protein. The encoded protein interacts with the proapoptotic member of the Bcl-2 family, Bcl-2-associated X protein (Bax) and may be involved in regulating apoptotic signaling pathways. This protein may also be involved in maintaining mitochondrial morphology. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]

Uniprot Description

SH3GLB1: May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion. Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1. Homodimer, and heterodimer with SH3GLB2. Binds BAX. Induction of apoptosis augments BAX binding. Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes. Belongs to the endophilin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Mitochondrial; Transferase; Apoptosis; EC 2.3.1.-

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: Golgi membrane; mitochondrial outer membrane; cytoplasm

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; lipid binding

Biological Process: positive regulation of protein oligomerization; apoptosis; protein oligomerization