Western Blot (WB)
(Western Blot Analysis: Jurkat +/-Insulin lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-phos-Bid-S65 (1:125). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Lane 1: Jurkat (-) insulin Lane 2: Jurkat (+) insulin Arrow indicates phospho-Bid-S65 (22kD))
Rabbit anti-Human BID Polyclonal Antibody | anti-BID antibody
BID, Phosphorylated (Ser61) (Bcl-2 Interacting Domain, BH3 Interacting Domain Death Agonist) (MaxLight 550)
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western Blot Analysis: Jurkat +/-Insulin lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-phos-Bid-S65 (1:125). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Lane 1: Jurkat (-) insulin Lane 2: Jurkat (+) insulin Arrow indicates phospho-Bid-S65 (22kD))
Western Blot (WB)
(Western Blot Analysis: Jurkat +/-Insulin lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-phos-Bid-S65 (1:125). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Lane 1: Jurkat (-) insulin Lane 2: Jurkat (+) insulin Arrow indicates phospho-Bid-S65 (22kD))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]
Uniprot Description
BID: a pro-apoptotic member of the Bcl-2 superfamily. Targets intracellular membranes and contains a BH3 death domain. Heterodimerizes with either the pro-apoptotic protein BAX or the anti-apoptotic protein BCL2, antagonizing its protective effect. The activity of BID is regulated by Caspase 8-mediated cleavage, exposing the BH3 domain and significantly changing the surface charge and hydrophobicity, causing translocation to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced variants have been found.
Protein type: Apoptosis; Mitochondrial
Chromosomal Location of Human Ortholog: 22q11.1
Cellular Component: mitochondrial outer membrane; membrane; mitochondrion; cytosol
Molecular Function: protein binding; ubiquitin protein ligase binding; death receptor binding
Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of apoptosis; apoptosis; positive regulation of protein homooligomerization; DNA damage response, signal transduction; response to estradiol stimulus; regulation of cell proliferation; positive regulation of protein oligomerization; neuron apoptosis; apoptotic mitochondrial changes; induction of apoptosis via death domain receptors; brain development; protein targeting to mitochondrion; protein homooligomerization
Research Articles on BID
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Product Notes
The BID bid (Catalog #AAA6465303) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BID, Phosphorylated (Ser61) (Bcl-2 Interacting Domain, BH3 Interacting Domain Death Agonist) (MaxLight 550) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BID can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the BID bid for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BID, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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