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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using BHLHE40 antibody.)

Rabbit anti-Human BHLHE40 Polyclonal Antibody | anti-BHLHE40 antibody

BHLHE40 antibody

Gene Names
BHLHE40; DEC1; HLHB2; BHLHB2; STRA13; Stra14; SHARP-2
Reactivity
Human
Applications
Western Blot, Immunofluorescence
Purity
Antibodies were purified by affinity purification using immunogen.
Synonyms
BHLHE40; Polyclonal Antibody; BHLHE40 antibody; DEC1; HLHB2; BHLHB2; STRA13; Stra14; SHARP-2; anti-BHLHE40 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of total BHLHE40 antibody.
Purity/Purification
Antibodies were purified by affinity purification using immunogen.
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
412
Applicable Applications for anti-BHLHE40 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
Western blotting: 1:500 - 1:2000
Immunofluorescence: 1:50 - 1:200
Immunogen Type
Recombinant Protein
Immunogen Description
Recombinant protein of human BHLHE40.
Target Name
BHLHE40
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using BHLHE40 antibody.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using BHLHE40 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using BHLHB2 antibody. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cell using BHLHB2 antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-BHLHE40 antibody
This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-box binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation.
Product Categories/Family for anti-BHLHE40 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
45,510 Da
NCBI Official Full Name
class E basic helix-loop-helix protein 40
NCBI Official Synonym Full Names
basic helix-loop-helix family, member e40
NCBI Official Symbol
BHLHE40
NCBI Official Synonym Symbols
DEC1; HLHB2; BHLHB2; STRA13; Stra14; SHARP-2
NCBI Protein Information
class E basic helix-loop-helix protein 40
UniProt Protein Name
Class E basic helix-loop-helix protein 40
UniProt Gene Name
BHLHE40
UniProt Synonym Gene Names
BHLHB2; DEC1; SHARP2; STRA13; bHLHe40; bHLHb2; DEC1; SHARP-2
UniProt Entry Name
BHE40_HUMAN

NCBI Description

This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-box binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation. [provided by RefSeq, Feb 2014]

Uniprot Description

BHLHB2: May function as a transcriptional factor to modulate chondrogenesis in response to the cAMP pathway.

Protein type: Nucleolus; Transcription regulation

Chromosomal Location of Human Ortholog: 3p26

Cellular Component: nucleoplasm; Golgi apparatus; nucleolus; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein domain specific binding; MRF binding; protein binding; protein homodimerization activity; protein heterodimerization activity; bHLH transcription factor binding; transcription corepressor activity; transcription factor activity

Biological Process: circadian rhythm; transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; negative regulation of transcription factor activity; circadian regulation of gene expression; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; entrainment of circadian clock by photoperiod

Research Articles on BHLHE40

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Product Notes

The BHLHE40 bhlhe40 (Catalog #AAA9408853) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BHLHE40 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BHLHE40 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Western blotting: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200. Researchers should empirically determine the suitability of the BHLHE40 bhlhe40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BHLHE40, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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