Rabbit BHLHE40 Polyclonal Antibody | anti-BHLHE40 antibody
BHLHE40 Antibody
Gene Names
BHLHE40; DEC1; HLHB2; BHLHB2; STRA13; Stra14; SHARP-2
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
BHLHE40; Polyclonal Antibody; BHLHE40 Antibody; BHLHB2; bHLHe40; Class B Basic Helix Loop Helix Protein 2; Class E basic helix loop helix protein 40; Clast5; DEC1; Differentially expressed in chondrocytes protein 1; E47 interaction protein 1; EIP1; Enhancer of split and hairy related protein 2; SHARP 2; Stimulated by retinoic acid gene 13 protein; STRA13; anti-BHLHE40 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
BHLHE40 Antibody detects endogenous levels of BHLHE40.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-BHLHE40 antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human BHLHE40.
Conjugation
Unconjugated
Post Translational Modifications
Ubiquitinated; which may lead to proteasomal degradation.Sumoylation inhibits its ubiquitination and promotes its negative regulation of the CLOCK-ARNTL/BMAL1 heterodimer transcriptional activator activity.
Subunit Structure
Homodimer. Heterodimer with BHLHE41/DEC2. Interacts with TCF3/E47. Interacts with ubiquitin-conjugating enzyme UBE2I/UBC9. Interacts with HDAC1, SUMO1, RXRA and ARNTL/BMAL1.
Tissue Specificity
Expressed in cartilage, spleen, intestine, lung, and to a lesser extent in heart, brain, liver, muscle and stomach.
Function
Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA and NR1H3/LXRA transactivation activity. May be involved in the regulation of chondrocyte differentiation via the cAMP pathway. Represses the transcription of NR0B2 and attentuates the transactivation of NR0B2 by the CLOCK-ARNTL/BMAL1 complex. Drives the circadian rhythm of blood pressure through transcriptional repression of ATP1B1 in the cardiovascular system.
Note
Predominantly localized in the nucleus (PubMed:11278694).
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,510 Da
NCBI Official Full Name
class E basic helix-loop-helix protein 40
NCBI Official Synonym Full Names
basic helix-loop-helix family, member e40
NCBI Official Symbol
BHLHE40
NCBI Official Synonym Symbols
DEC1; HLHB2; BHLHB2; STRA13; Stra14; SHARP-2
NCBI Protein Information
class E basic helix-loop-helix protein 40; basic helix-loop-helix domain containing, class B, 2; class B basic helix-loop-helix protein 2; differentially expressed in chondrocytes 1; differentially expressed in chondrocytes protein 1; differentiated embry
UniProt Protein Name
Class E basic helix-loop-helix protein 40
Protein Family
UniProt Gene Name
BHLHE40
UniProt Synonym Gene Names
BHLHB2; DEC1; SHARP2; STRA13; bHLHe40; bHLHb2; DEC1; SHARP-2
UniProt Entry Name
BHE40_HUMAN
Similar Products
Product Notes
The BHLHE40 bhlhe40 (Catalog #AAA9629790) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BHLHE40 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BHLHE40 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the BHLHE40 bhlhe40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BHLHE40, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
